Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.3.1 (alkaline phosphatase)
47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

3 preparations of 17beta-estradiol and norethisterone acetate were administered to 34 climacteric and 175 postmenopausal women to treat climacteric symptoms and symptoms of estrogen deficiency. 56 women were treated with trisekvens (Group 1), 131 with trisekvens forte (Group 2), and 22 with estrofem forte (Group 3). Triglycerides, cholesterol, calcium, sodium and potassium ions, alkaline phosphatase, creatinine, glucose, protein, albumin, haptoglobin, zinc sulphate, iron, TIBC, bilirubin, ALAT and ASAT, and follicle stimulating hormone (FSH), luteinizing hormone (LH), and low polar estrogens (LPE) were measured. All patients exhibited lowered S-cholesterols which reverted to normal after 6 months treatment. S-triglycerides were unchanged except in Group 1 patients where there was a slight increase after 24 months use (p .01). Serum FSH and LH decreased during treatment and this decrease was most pronounced in Groups 2 and 3. Serum LPE levels increased in Group 1, for climacteric women, to normal luteal values and in postmenopausal women to proliferation values. In groups 2 and 3, serum LPG for postmenopausal women reached luteal values. Climacteric symptoms disappeared with therapy and there was an improvement in symptoms caused by estrogen deficiency. 34 patients discontinued treatment, 14 changing to another preparation. These preparations were well tolerated with few side effects.
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PMID:Treatment of climacteric and postmenopausal women with 17-beta-oestradiol and norethisterone acetate. 60 3

Holotyrosine phenol-lyase (EC 4.1.99.2), a pyridoxal-5'-phosphate (PLP)- requiring enzyme, was shown to rapidly dissociate when injected into BDF1 mice. The holoenzyme dissociated when incubated in plasma but not 0.01 M potassium phosphate (pH 7.4) buffer at 37 degrees C. A nonspecific alkaline phosphatase from calf intestine was found to inactivate the holoenzyme at pH 7.4 and 37 degrees C. This inactivation was inhibited in the presence of 0.5 M potassium phosphate buffer. Two other PLP-requiring enzymes, aspartate aminotransferase (EC 2.6.1.1), and alanine aminotransferase (EC 2.6.1.2) were inactivated by alkaline phosphatase in a similar manner. Incubation of holotyrosine phenol-lyase in the presence of bovine serum albumin also resulted in a reduction of holoenzyme activity but partially protected the enzyme from inactivation by alkaline phosphatase. A nuclear fraction having PLP-hydrolyzing activity also inactivated holotyrosine phenol-lyase. A regulatory function for alkaline phosphatase in the metabolism of PLP-requiring enzymes is suggested by these data.
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PMID:Albumin and alkaline phosphatase as factors involved in the regulation of tyrosine phenol-lyase activity. 65 5

The authors report on biochemical and hematologic investigations during 6 extracorporeal heterologous perfusions by means of porcine livers in 4 patients suffering from acute hepatic failure. The findings stress the functioning of porcine liver in the extracorporeal system. All livers produced bile with higher values of bilirubin and pH than found in serum. At each perfusion the serum levels of bilirubin decreased. Activities of serum enzymes (GOT, GPT, GLDH, gamma-GT, alkaline phosphatase) and the levels of potassium and sodium behaved differently. This could be explained by different damages of the porcine livers during perfusion which always occur. In 3 perfusions decreased leucocyte counts were observed, explicable immunologically. Extracorporeal heterologous perfusion by means of an alien liver is considered a possible way for temporary replacement of liver function and for removing toxins from the host. Success in single cases with reasonable indication are considered possible. The patients endured the perfusions well. After transitory improvement of their general conditions they succumbed from their severe diseases, though.
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PMID:[Biochemical and hematological parameters during extracorporeal heterologous perfusion with a porcine liver in acute liver failure]. 65 73

A modified Roux-en-y repositioning of rat proximal small intestine resulted in a gut segment (A) exposed only to digestive secretions, but not to food and a gut segment (B) exposed to food, stomach juice and by reflux only to digestive secretions, and a third segment (C) exposed to both, food and digestive secretions. The changes in segment A were qualitatively very similar to those occurring after removal of luminal nutrition (intravenous feeding, self-emptying blind loop, and Thiry Vella loop). These findings support the hypothesis that the presence of luminal nutrition is a major factor regulating mucosal mass and enzyme activity in rat proximal small intestine. The changes in the luminal environment in segment B caused an increase in mucosal mass (in the proximal half only), an increase in sucrase activity which paralleled the increase in mucosal mass, and no change in activity of alkaline phosphatase which in fact was a decrease in activity ;at the cellular level'. Later on the net absorption of sodium and potassium was improved and the disappearance of galactose was unchanged when referred to unit length of small intestine.In segment C there was a small increase in mucosal mass, an increase in activity only for alkaline phosphatase, and an improvement of the net absorption of sodium without changes in the disappearance of galactose. These changes were compatible with a more proximal promotion of a distal gut segment.
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PMID:Analysis of the effects of food and of digestive secretions on the small intestine of the rat: III. Mucosal mass, activity of brush border enzymes, and in vivo absorption of galactose, sodium, and potassium. 68 Jun 2

The influence of circulatory conditions, point of blood withdrawal (arterial, central or peripheral venous) and the plasma-serum relation on 29 clinical chemical and hematological parameters were studied with 22 polytraumatized patients. The conditions studied significantly affect the results, and must be taken into consideration in evaluating the results and their comparison to reference values. This is especially important for the determination of the catalytic activities of creatine kinase, aspartate and alanine aminotransferases, and alkaline phosphatase in centralized-circulatory patients, for the total protein the electrophoretic fractions and analyses of blood gas as a function of the point of blood withdrawal, and for the total protein, gamma-globulins and potassium when plasma is analysed instead of serum.
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PMID:[Influence of specimen withdrawal on the results of chemical analyses of blood, plasma and serum in patients with stable or centralized circulation (author's transl)]. 69 May 67

The results of sending specimens through a computerized pneumatic airtransport system and manually delivering specimens were compared for 15 chemical tests and six hematologic procedures. All specimens were collected from inpatients and outpatients into evacuated glass containers. The specimens traversed a maximum of 829 feet (253 meters) involving 16 bends and eight transfer units at 25 feet/second (7.6 meters/second). Only the activity of lactate dehydrogenase exceeded the precision of the test in pneumatically transported specimens. Ruptured erythrocytes in incompletely filled vacuum tubes were the likely source of the increased lactate dehydrogenase activity. Neither the serum sodium, potassium, chloride, carbon dioxide, total protein, albumin, calcium, glucose, creatinine, total bilirubin, alkaline phosphatase, aspartate transaminase, acid phosphatase, uric acid, leukocyte count, erythrocyte count, hemoglobin, hematocrit, nor the prothrombin time and partial thromboplastin time were affected by pneumatic transport. It is concluded that the pneumatic system tested provides a safe, efficient method of transporting the blood specimens tested.
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PMID:Evaluation of a computer-directed pneumatic-tube system for pneumatic transport of blood specimens. 70 6

Furosemide is frequently used for ascites and causes adverse reactions (AR). In an intensive prospective drug monitoring study of 1,920 patients, 172 (8.9%) had cirrhosis of the liver and received furosemide. Mean age was 53 years, and 66.3% were male; and 87% had alcoholic cirrhosis. Eighty-eight (51.2%) had 221 events that by consensus of the monitoring team and attending physicians were either definitely of probably related to furosemide. No AR was fatal but 24% of patients had severe reactions. Almost all reactions were dose-related (96%). The most common were electrolyte disturbances (23.3% of patients) and volume depletion (14%). Furosemide-induced coma occurred in 20 (11.6%) patients and was more frequent in patients with prior hepatic encephalopathy (p less than 0.0005). Higher total doses (p less than 0.001), hyerbilirubinemia (p less than 0.05), prolonged prothrombin time (p less than 0.02), and longer hospital stay (p less than 0.001) were associated with higher frequencies of AR to furosemide. The frequency of hypokalemia did not decrease when potassium chloride or potassium-sparing diuretics were added to furosemide therapy. Frequdncy of AR did not correlate with age, sex, renal impairment, serum albumin, transaminase, or alkaline phosphatase.
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PMID:Furosemide-induced adverse reactions in cirrhosis of the liver. 75 67

Oral glucose tolerance curves were constructed for 50 normal pregnant women, 25 pre-diabetics and 25 cases of diabetic pregnant women of different degrees of disturbance in carbohydrate metabolism. The mean rise in blood glucose level after the intake of 50 g glucose, was 53% in normal cases, and only 41% in the prediabetic cases, due to increased insulin secretion in the prediabetic group. This group of pregnants gave flat-topped curves in 60% of the cases studied, as compared to 10% of the normal pregnant cases in the same study. This was attributed to delayed insulin secretion in the prediabetic group. On the other hand, all diabetic cases gave peaked glucose tolerance curves during the first hour after glucose intake. The results of insulin-glucose tolerance test, effect of fasting for four hours and tolbutamide sensitivity test suggested that the prediabetic pregnants showed hyperinsulinemia which became evident during fasting. The pancreas of the prediabetic responds more readily, by the release of insulin, to the stimulus of oral hypoglycemic agents, such as sodium tolbutamide, than in the case of normal pregnants. Measurements of serum inorganic phosphorus, serum potassium and serum alkaline phosphatase of these 3 groups of pregnants, showed a definite disturbance in the liver function of the prediabetics which became more obvious in the diabetic group.
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PMID:Bood glucose level in pregnancy. 78 19

Methods have been developed for the isolation on a semi-micro scale of a plasma membrane-enriched fraction from rat islets of Langerhans. An important feature of these experiments is the use of 125I-labeled wheat germ agglutinin as a specific probe for plasma membrane-containing fractions. The partly purified plasma membrane fraction had a density in sucrose of about 1.10 and was enriched in the activities of 5'-nucleotidase, alkaline phosphatase, sodium-potassium, and magnesium-dependent ATPase and adenylate cyclase. It contained only very low levels of acid phosphatase, cytochrome c oxidase, insulin, and RNA. Further purification was hampered by the relatively small amounts of fresh plasma membrane material that could be obtained from 16-24 rats in each experiment. When islets were prelabeled with radioactive fucose, the plasma membrane-enriched fraction contained radioactivity at a four- to fivefold higher specific acivity than the whole islet homogenate. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis of plasma membrane-enriched fractions pooled from several experiments revealed a distinctive pattern of protein bands as compared with other less pure fractions. With respect to rapidity, apparent specificity, and easy reversibility of the labeling of the plasma membrane fraction, 125I-wheat germ agglutinin provides a highly useful tool for the detection of microgram quantities of plasma membrane components which should be applicable to many other systems as well.
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PMID:Preparation and characterization of plasma membrane-enriched fractions from rat pancreatic islets. 79 56

Tartar emetic (potassium antimony tartrate) has been used since a long time as the drug of choice for the treatment of Bilharziasis in Egypt. This drug, though effective, has severe side effects. A newly synthesized trivalent antimony preparation (piperazine di-antimonyl tratrate) Bilharcid, has proved in animal experiments to be less toxic, more effective, and having little side effects. The drug was tried in various schemes on various age groups of patients infected with S. haematobium. Control cases were treated with tartar emetic. Urine analysis was done for the detection of living or dead ova before and after treatment. E.C.G., alkaline phosphatase, serum transaminase, lactic dehydrogenase, and urea tests were done before and after treatment. Follow up studies were recorded for three months after treatment. Results are presented in the full text.
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PMID:Preliminary report on some clinical and biochemical observations in patients treated with Bilharcid. 81 Mar 41


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