Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.3.1 (alkaline phosphatase)
47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of zinc pyrithione (ZnPT) and sodium pyrithione (NaPT), including the influence of various vehicles, upon whole blood and plasma zinc levels and serum alkaline phosphatase (SAP) have been investigated in rabbits following dermal and/or iv administration. Two such vehicles, ammonium lauryl sulfate (ALS) and triethanolamine lauryl sulfate, affected zinc homeostasis differently than the pyrithiones, in that, unlike the pyrithiones, no whole blood changes were observed, although there were delayed and sustained declines in plasma zinc and SAP values. These changes were most likely related to the skin irritation caused by the surfactants. In contrast, NaPT-dimethyl sulfoxide (DMSO) dermal and iv exposures produced rapid decreases in plasma zinc followed by quick recovery, coupled with smaller and unsustained declines in SAP. Large increases in whole blood zinc were also observed in both cases, as well as in a ZnPT-DMSO iv exposure. DMSO itself had no effects on the measured parameters. Experiments involving combinations of the pyrithiones and ALS demonstrated effects on zinc homeostasis that were attributable to both substances, i.e. large increases in whole blood zinc (PT effect), quick drops in plasma zinc (PT effect) and slowly recovering plasma zinc and SAP values (surfactant effect). The chelating nature of the PT molecule may have been responsible for some of the observed changes in zinc distribution.
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PMID:Effects of pyrithiones and surfactants on zinc and enzyme levels in rabbits. 739 40

We have recently demonstrated that phenytoin is an osteogenic agent at low doses. The present paper describes observations that a mitogenic dose (i.e., 20 microM in BGJb medium) of fluoride significantly augments the phenytoin-dependent stimulation of normal human bone cell proliferation and alkaline phosphatase (ALP) activity in cell culture. Additionally, the present study was designed to investigate whether fluoride and phenytoin would interact to increase bone formation in rats in vivo. Four groups of weight-matched adult male rats received daily I.P. injection of (1) vehicle (10% DMSO), (2) 5 mg/kg/day phenytoin, (3) 5 mg/kg/day phenytoin and 50 ppm NaF, and (4) 50 ppm NaF and vehicle, respectively, for 36 days. Sodium fluoride (NaF) was delivered in drinking water. Blood samples were drawn weekly and analyzed for serum osteocalcin, ALP, calcium, phosphorus, and 25(OH)D3. Rats were labeled with tetracycline at day 21 and 30 and histomorphometric analysis was carried out on the tibia at the end of the experiment. Neither agent by itself or together affected the serum calcium, phosphorus, or 25(OH)D3 levels. All measures of bone formation, i.e., serum osteocalcin level and ALP activity, bone ALP specific activity, mineral apposition rate, bone formation rate, and % bone formation surface, were increased by each agent. Fluoride and phenytoin together produced bigger increases in each parameter than did each agent alone. Trabecular bone volume was increased in the tibial metaphysis by fluoride or phenytoin alone; and when administered together, the two agents produced a greater increase.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Phenytoin and fluoride act in concert to stimulate bone formation and to increase bone volume in adult male rats. 762 47

The in vitro effects of dimethyl sulfoxide (DMSO) on MGC 80-3 cells were studied by light microscopy, transmission electron microscopy and biochemical methods. The results indicated that the inhibitory effects of DMSO on the growth of MGC 80-3 cells was concentration dependent and 1.5% DMSO was suitable in the present study. The growth rate, mitotic index, colony forming efficiency and Con A-aggregation of the cells treated with 1.5% DMSO in vitro for 7 days was reduced respectively by 35.15%, 18/1000, 90% and 55.8%. It was remarkable that the activity of membrane-associated alkaline phosphatase, which is not presented in normal human gastric mucosa, was decreased by 90% in the treated cells. There was a 75% decrease of the rate of tumorigenesis in the treated cells as compared with the tumorigenic rate of the untreated MGC 80-3 cells inoculated into nude mice. Gross morphological changes of MGC 80-3 cells treated with 1.5% DMSO were evident including enlargement and flatness of the cells, stumpy microvillies instead of long and rigid ones, and extremely well-developed Golgi apparatus and rough endoplasmic reticulum. This data indicated that DMSO was able to induce differentiation of MGC 80-3 cells and change their malignant phenotypes.
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PMID:[DMSO induced differentiation of human gastric adenocarcinoma cell line MGC 80-3]. 780 21

The differentiation of HL-60 promyelocytic cells toward mature granulocytic cells induced by dimethyl sulfoxide (DMSO) was accompanied by a quantitative similar increase in alkaline phosphatase (ALP) activity and decrease in protein kinase C (PKC) activity. The combination of DMSO and sphinganine (SP), a potent inhibitor of PKC, increased in parallel the percentage of mature cells and the ALP activity. The enhancement of DMSO-induced differentiation and the potentiation of the decrease of PKC activity by SP also seemed to correlate with each other. Our results indicate that both ALP and PKC may play a role in the DMSO-induced granulocytic differentiation.
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PMID:Sphinganine potentiation of dimethyl sulfoxide-induced granulocyte differentiation, increase of alkaline phosphatase activity and decrease of protein kinase C activity in a human leukemia cell line (HL-60). 813 36

This investigation was undertaken to determine if an interaction of toxicologic importance might occur during a prolonged exposure of rats to carbon tetrachloride (CCl4) and Dimethyl Sulphoxide (DMSO). CCl4 administration produced a significant decrease in hepatic microsomal glucose-6-phosphatase activity accompanied by a small increase in alkaline phosphatase activity, Glutathione depletion was highest when CCl4 was administered alone. DMSO, did not increase hepatic uptake of glucose. These findings suggest that DMSO given at low dose can prevent the decrease of hepatic glucose-6-phosphatase but may indirectly affect the level of tissue glucose.
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PMID:The effect of dimethyl sulphoxide on CCl4-induced damage to the liver and its effects on hepatic glutathione and glucose. 871 61

A low concentration of differentiation inducers such as dimethylsulphoxide (DMSO), sodium butyrate, hexamethylene bisacetamide and sodium phenylacetate greatly enhanced the antiproliferative effect in vitro and in vivo of interferon alpha (IFN-alpha) to several human lung adenocarcinoma cells. The agents induced morphological changes in the adenocarcinoma cells and the agents together with IFN-alpha-induced alkaline phosphatase activity, which is a typical marker of type II pneumocyte maturation. To understand the mechanism of the DMSO-enhanced interferon sensitivity, we examined the effect of DMSO on high-affinity IFN-alpha receptor and interferon-stimulated promoter-binding factors. The lung adenocarcinoma cells were not impaired in IFN-alpha receptor and interferon-stimulated gene transactivation factor 3 (ISGF-3). Our data suggest that the enhancement of interferon sensitivity in the lung adenocarcinoma cells acts downstream of the activation of ISGF-3.
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PMID:Enhancement of sensitivity of human lung adenocarcinoma cells to growth-inhibitory activity of interferon alpha by differentiation-inducing agents. 876 68

A differentiated human colonic epithelial cell has undergone relatively stable molecular, biochemical, and cellular alterations resulting in the acquisition of structures, activities, and functions that characterize it as one of at least three mature phenotypes: a columnar absorptive, secretory, or enteroendocrine cell. We have shown previously that induction of HT29 cells with the short-chain fatty acid sodium butyrate elevates alkaline phosphatase activity, a marker of the absorptive cell phenotype, and increases mitochondrial gene expression. Furthermore, this induction is accompanied by subsequent apoptosis and cell shedding. In this report, we have investigated the effects of forskolin, a potent inducer of the MUC2 gene in HT29 cells, a marker of the secretory phenotype, and have shown that neither apoptosis nor mitochondrial gene expression are significantly stimulated. Thus, differentiation along the secretory cell lineage may not play a major role in apoptosis of colonic epithelial cells. Moreover, we have also investigated two polar solvents, DMSO and dimethylformamide, which have been reported to induce a more differentiated, but as yet not well characterized, phenotype in colonic carcinoma cells in culture. Although neither polar solvent induces alkaline phosphatase expression or MUC2 expression, both induce significant apoptosis, again associated with significant elevation of mitochondrial gene expression. Thus, elevation of mitochondrial gene expression appears to be an important pathway in the induction of apoptosis in colonic epithelial cells in culture, whether or not markers characteristic of differentiation along either the absorptive or secretory cell lineage are induced.
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PMID:Steady-state levels of mitochondrial messenger RNA species characterize a predominant pathway culminating in apoptosis and shedding of HT29 human colonic carcinoma cells. 878 38

Complex chemical mixtures at hazardous waste sites can potentially consist of a marker chemical and several other chemicals, each of which can have different modulating actions on the dermatotoxicity of the marker chemical and/or other components in the mixture. A total of 16 mixtures, consisting of a marker chemical direct red 28 (DR28), a solvent (80% acetone or DMSO in water), a surfactant (0 or 10% sodium lauryl sulfate, SLS), a vasodilator (0 or 180 microg methyl nicotinate, MN) and a reducing agent (0 or 2% stannous chloride, SnCl2) were selected. Isolated perfused porcine skin flaps (IPPSFs), which have been proven to be an in vitro model for assessing absorption and toxicity, were utilized. These mixtures did not cause severe dermatotoxicity. However, light microscopic observations depicted minor alterations (intracellular and intercellular epidermal edema) with DMSO mixtures than with acetone mixtures. The presence of SLS caused an alteration in the stratum corneum. Enzyme histochemical staining for alkaline phosphatase (ALP) and nonspecific esterase (NSE) revealed no significant treatment effects, but increased staining for acid phosphatase (ACP) in the stratum basale was significant when associated with SLS or SLS + MN in DMSO mixtures. At 8 h post-dose, only DMSO mixtures containing SL + MN, SL + SnCl2, or SLS + MN + SnCl2 significantly increased transepidermal water loss. In conclusion, this study demonstrated that various mixtures, especially those containing SLS alter the epidermal barrier differently with complex interactions occurring simultaneously.
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PMID:Cutaneous toxicity of the benzidine dye direct red 28 applied as mechanistically-defined chemical mixtures (MDCM) in perfused porcine skin. 948 52

Alterations in protein tyrosine phosphate (PTP), lactate, and fructose 2,6-bisphosphate (F-2,6-P2) levels have been associated with induced MEL cell differentiation and commitment to terminal cell division (TCD). The possible relationships of perturbations in PTP metabolism and reduction in lactate formation during differentiation were investigated utilizing sodium orthovanadate, Na3VO4, primarily an inhibitor of PTP phosphatases, and levamisole, considered an alkaline phosphatase inhibitor. Both of these compounds were found to effectively inhibit the TCD-associated differentiation induced by DMSO, HMBA, and Na butyrate and to abrogate the differentiation-associated reduction in lactate accumulation due to these agents. However, they were found not to inhibit hemin-induced hemoglobin synthesis which is independent of TCD and does not alter lactate metabolism. Two brominated levamisole analogs, L-p-bromotetramisole and D-p-bromotetramisole, were also found to be inhibitors of TCD-associated differentiation and to be effective at even lower concentrations than levamisole. The changes in TCD-associated differentiation and lactate production exhibited the same concentration-dependence with respect to the inhibitors. These findings strengthened the theory that TCD-associated differentiation, decreased lactate production, and sensitivity to phosphatase inhibitors are all associated. Since the induction of MEL cell differentiation has been shown to be associated with, and is thought to be due to, the induction of PTP phosphatase activity and Na3VO4 is thought to inhibit the differentiation by inhibiting PTP phosphatase activity, the effect of levamisole on PTP levels was determined. Levamisole, like Na3VO4, was found to increase the tyrosine phosphate levels of proteins of similar molecular weights in intact cells in both the presence and absence of a differentiation inducer. Several phosphotyrosine-containing, similarly sized proteins were particularly affected by differentiation induction and by Na3VO4 and levamisole treatment. Changes in the levels of tyrosine phosphate-containing proteins of approximately 92-96, 60, and 38 kd were particularly noticeable. The induction of differentiation reduced PTP levels and inhibition of differentiation due to treatment with either Na3VO4 or levamisole increased their levels. These data suggest relationships between signal transduction pathways involved in differentiation and TCD, the regulation of lactate and F-2,6-P2 metabolism, and PTP levels.
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PMID:The phosphatase inhibitors, orthovanadate and levamisole, inhibit induction of erythroid differentiation and abrogate the associated inhibition of glycolysis. 953 18

The promutagenic base 8-hydroxy-2'-deoxyguanosine (8-OH-dG) in DNA is known to be formed from oxygen radical attack on 2'-deoxyguanosine (dG) as a result of oxidative stress. Formation of 8-OH-dG from dG during workup is strongly dependent on temperature and transition metals and is mediated by oxygen radicals. The 8-OH-dG formation at temperatures between 0 and 140 degrees C for 1.5 h in an "ultrapure" solution followed a third-order equation. Fe2+ in the nM range mediated the formation of 8-OH-dG from dG without addition of H2O2. Fe3+, Cu+, and Cu2+ were shown to have weaker oxidative effects in comparison to Fe2+. The pH (5.0-9.0) had a very limited effect on 8-OH-dG formation. Acid phosphatase, which contains iron at its active site, caused the formation of 8-OH-dG, whereas alkaline phosphatase did not. Phenol was not found to be oxidative. Fe2+-catalyzed formation of 8-OH-dG was completely blocked by the nitroxide 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO), whereas DMSO, mannitol, and DMPO had a significantly weaker protecting effect. Catalase cleaved the dG molecule and was not suitable for use. A simple, fast, and inexpensive method for 8-OH-dG workup and analysis was developed, and the background level seen in liver from 13-week-old male Sprague-Dawley rat was 0.23 +/- 0.020 8-OH-dG/10(5) dG, which is up to 200 times lower than reported values from some other methods and up to 26 times lower when compared to other reports using HPLC-EC methods. In summary, the TEMPO method reduces oxidation of dG to 8-OH-dG during workup by (1) using chemicals low in transition metals, (2) using a cold workup procedure, (3) limiting the incubation time, and (4) using the nitroxide TEMPO in all steps.
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PMID:Reduction of oxidation during the preparation of DNA and analysis of 8-hydroxy-2'-deoxyguanosine. 970 49


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