Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Exposure of laying hens to an acute heat stress period (38 degrees C) produced a decrease (P < or = 0.05) in blood plasma magnesium compared with pre-heat stress (23 degrees C) levels. Blood plasma glucose, alkaline phosphatase, total protein, uric acid and creatinine were not changed (P > or = 0.05) by exposure to 38 degrees C compared with the first 23 degrees C exposure. Inorganic phosphorus, calcium, potassium and sodium levels were not affected by acute heat-stress exposure or carbon dioxide addition. These results suggest that acute heat stress had no dramatic effect on plasma enzymes, metabolites and electrolytes of laying hens.
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PMID:Laying hen responses to acute heat stress and carbon dioxide supplementation: II. Changes in plasma enzymes, metabolites and electrolytes. 755 28

To investigate the mechanism of biological calcification in vitro, a model system consisting of an acrylamide gel block (1 x 3 x 3 mm) and fetal bovine serum was developed. Mineral deposition was induced in gel blocks which were immersed in 300 microliters of fetal bovine serum at 37 degrees C for 7 days in a CO2 incubator. X-ray diffraction indicated that the mineral was hydroxyapatite with low crystallinity. Effects of the concentration of acrylamide gel, the partial pressure of CO2 and matrix proteins within the gel on the mineral formation were investigated. In the gel concentration range of 10-60%, the largest amount of crystal grew in 40% acrylamide gel, where the serum protein did not penetrate. With an increase in the partial pressure of CO2 the Ca content in the gel block increased, reached the highest level at about 3.5% CO2 and then began to decrease. In 40% gel and at 5% CO2, the mineral formation was enhanced by phosvitin, phosphophoryn, demineralized dentin powder and alkaline phosphatase. Mineral deposition occurred around the collagen fibers immobilized in 40% acrylamide gel. These results indicate that 1) a putatively serum-derived inhibitor of calcification with high-molecular weight was prevented from penetrating into the 40% acrylamide gels, 2) immobilized polyanionic proteins and alkaline phosphatase were able to increase mineral deposition and 3) the partial pressure of CO2 greatly influenced the mineral deposition. It was concluded that this gel system is useful to investigate the mechanism of biological calcification in vitro.
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PMID:A new method for in vitro calcification using acrylamide gel and bovine serum. 755 52

Blood samples were taken and subjected to biochemical analysis in the crossbred cows of the Red-Pied, Black-Pied or Holstein-Friesian breed raised on a large farm under standard conditions, with the average annual milk yield of 4,300 kg milk, and divided into two groups--cows with afterbirth retention and without it; the samples were taken in the last period of pregnancy (since day 245), during parturition, and within the first 50 days post partum. In both groups, the values of acid-base balance and metabolic profile mostly ranged within the interval of reference values, nevertheless there were certain trends and differences in absolute values as well as in the dynamics of changes, but they did not always show a doubtless character and the same significance. The cows with afterbirth retention exhibited a trend of a more expressive decrease in partial pressure CO2, actual acid output and phosphorus level. Glucose level at the end of pregnancy was statistically significantly lower (P < 0.01), cholesterol level also decreased (P < 0.05). On the other hand, the cows with afterbirth retention had, at the end of pregnancy, statistically significantly higher concentrations of urea and creatinine (P < 0.05-0.01), a higher bilirubin level and enzyme activities of acid phosphatase, and particularly of aspartate aminotransferase and lactate dehydrogenase (P < 0.05-0.01). There were insignificant differences in the concentrations of total protein, calcium, magnesium and enzyme activity of alkaline phosphatase and gammaglutamyl transferase.
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PMID:[Metabolic profile in cows in the peripartum period with and without retained placenta]. 757 Dec 42

We used the CO2 laser (group 1) and conventional microsurgery (group 2) for anastomosis of the freshly divided uterine horns of rats and compared the two methods. Each group was then compared with a control group in whom only exploration was carried out at laparotomy. Comparison was done regarding the clinical and histologic results. In addition, serum levels and tissue concentrations of alkaline phosphatase (ALP) and lactic dehydrogenase (LDH) were measured, and the three groups were compared. No significant difference was found between the mean adhesion scores of groups 1 and 2; however, when the control group was compared with the other groups, the differences were statistically significant. The tubal patency rates in groups 1 and 2 and the control group were 83.3%, 79.2% and 100%, respectively, and the pregnancy rates in those groups were 54.5% (6/11), 45.5% (5/11) and 100% (10/10). The differences in tubal patency and pregnancy rates between groups 1 and 2 were not significant, but when each was compared with the control group, the differences were significant. The mean scores for mucosal regeneration and disruption of the muscularis layer in group 1 were significantly lower than those in group 2. Serum levels and tissue concentrations of ALP and LDH in the control group were lower than in groups 1 and 2, and the differences between the control group and each of the other groups were significant; however, no significant difference was found between groups 1 and 2.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Anastomosis of the freshly divided uterine horns of rats with the CO2 laser vs. microsurgery. 772 77

Nine yearling crossbred beef heifers, Bos taurus L., were used to examine physiological responses to horn fly, Haematobia irritans (L.), infestation. Heifers were stanchioned indoors in individual environmentally controlled rooms. On day 0, each animal received 0, 500, or 1,000 horn flies. Fly numbers were adjusted daily to maintain an appropriate infestation for each heifer. Feed intake, respiration rate, and rectal temperature was recorded daily. In addition, blood samples were collected from each animal on days 0, 12, and 33 for serum constituent analysis. To monitor metabolic hormone status, intensive blood samples were also collected hourly for 6 h on days 0, 12, and 33. Throughout the period of treatment, feed intake values were similar among treatments resulting in comparable body weight at the end of the trial. Respiration rates on each day were similar among groups. Rectal temperature was also unaffected by horn fly infestations. Serum electrolyte analysis revealed that neither Na, K, Cl, Ca, P, nor Fe differed among treatments. Likewise, HCO3/CO2 ratio, anion gap, and serum osmolality did not differ among treatment groups. Major indicators of nutrient status (glucose, cholesterol, triglycerides, urea N, creatinine, uric acid, albumin, globulin) and insulin, growth hormone, and prolactin were also unaffected. Serum bilirubin and enzyme (alkaline phosphatase, creatine phosphokinase, gamma-glutamyl transpeptidase, lactic dehydrogenase, aspartate amino transferase, alanine amino transferase) concentrations were similar in control heifers and those infested with horn flies.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Serum constituent profiles of beef heifers infested with horn flies (Diptera: Muscidae). 783 15

When bone is cultured in acidic medium produced by a reduced bicarbonate concentration ([HCO(3-)]), a model of metabolic acidosis, there is greater net calcium efflux than when the same decrement in pH is produced by an increased partial pressure of carbon dioxide (PCO2), a model of respiratory acidosis. To determine the effects of metabolic and respiratory acidosis on bone cell function we cultured neonatal mouse calvariae for 48 h under control conditions (pH approximately 7.40, PCO2 approximately 41 mmHg, [HCO(3-)] approximately 25 meq/l) or under isohydric acidic conditions simulating metabolic (pH approximately 7.09, [HCO(3-)] approximately 12) or respiratory (pH approximately 7.10, PCO2 approximately 86) acidosis and measured osteoblastic collagen synthesis and alkaline phosphatase activity and osteoclastic beta-glucuronidase activity. Collagen synthesis was inhibited by metabolic (23.2 +/- 1.3 vs. 30.3 +/- 1.0% in control) but was not altered by respiratory (32.3 +/- 0.6) acidosis. Alkaline phosphatase activity was inhibited by metabolic (402 +/- 16 vs. 471 +/- 15 nmol P.min-1.mg protein-1 in control) but not altered by respiratory (437 +/- 25) acidosis. beta-Glucuronidase activity was stimulated by metabolic (1.02 +/- 0.06 vs. 0.78 +/- 0.05 micrograms phenolphthalein released.bone-1.h-1 in control) but not altered by respiratory (0.73 +/- 0.06) acidosis. Net calcium efflux in control was increased by metabolic (783 +/- 57 vs. 20 +/- 57 nmol.bone-1.48 h-1 in control) and by respiratory (213 +/- 45) acidosis; however, calcium efflux with metabolic was greater than with respiratory acidosis.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Stimulated osteoclastic and suppressed osteoblastic activity in metabolic but not respiratory acidosis. 784 Jan 63

Subclinical intoxication of livestock with Astragalus and Oxytropis species (locoweeds) results in decreased animal feed conversion, reduced weight gains, and reproductive failure. Sensitive diagnostic methods to definitively diagnose and monitor intoxication are needed to minimize these losses and better manage locoweed-infested pastures and rangelands. Sera from cattle grazing locoweed were evaluated for alpha-mannosidase activity, serum biochemical values, electrolytes, and thyroid hormone concentrations. As the cows began to ingest locoweed, the mean serum alpha-mannosidase activities dropped significantly (400.0 microM to 72.5 microM). Changes in other serum chemistry values were less specific; however, individual animals (generally those ingesting more locoweed) had elevated levels of alkaline phosphatase (ALP), aspartate aminotransferase, and lactate dehydrogenase, with decreased serum total protein (5.8 +/- 0.8 g/dl) and albumin (2.3 +/- 0.3 g/dl). Mean serum thyroid concentrations (both T4 and T3) were lower in animals that were ingesting locoweed. The calculated swainsonine dose correlated statistically with serum alpha-mannosidase activity, ALP, albumin, Cl, CO2, and thyroid hormone T3. This correlation suggests that serum alpha-mannosidase activity along with potential changes in ALP, albumin, and thyroid hormone concentrations is a sensitive indicator of locoweed exposure and intoxication. These parameters may also be useful for monitoring intoxication and allowing subclinically affected cattle to be removed from infested areas before irreversible damage occurs.
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PMID:Serum alpha-mannosidase activity and the clinicopathologic alterations of locoweed (Astragalus mollissimus) intoxication in range cattle. 785 27

During growth of streptococci of Lancefield groups A and C in a culture medium containing glucose, yeast extract and peptone, two main growth phases occur: growth phase I and growth phase II (diauxic growth). They are separated by a short stationary phase (1st stationary phase). The diauxic growth is caused by transient limitations as well as the availability of new sources of the amino acids L-serine and L-arginine. Growth phase I consists of an exponential and a nearly linear part. These growth kinetics are reflected by the kinetics of gas metabolism as well as by product formation. Hyaluronic acid is formed during the nearly linear phase whereas the enzyme alkaline phosphatase, is exclusively excreted in the 1st stationary phase. Also carbon dioxide and L-lactate are mainly produced in a growth phase-dependent mode. In the late stationary phase (2nd stationary phase) more oxygen is consumed whereas the demand for oxygen in the 1st stationary phase is nearly zero.
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PMID:Kinetics of growth and product formation in cultures from streptococci of groups A and C. 785 43

The effect of concanavalin A (Con A) on maturing and terminal differentiation in permanent chondrocyte cultures were examined. Chondrocytes isolated from permanent cartilage were seeded at low density and grown in MEM medium containing 10% fetal bovine serum, 50 micrograms/ml of ascorbic acid and antibiotics, at 37 degrees C under 50% CO2 in air. At 0.3% of low serum concentration, addition of Con A to the culture medium increased by 3- to 4-fold the incorporation of [35S] sulfate into large chondroitin sulfate proteoglycan that characteristically found in cartilage. Chemical analysis showed a 4-fold increase in the accumulation of macromolecular containing hexuronic acid in Con A-maintained cultures. The effect of Con A on [35S]sulfate incorporation into proteoglycan was greater than that of various growth factor or hormones. Brief exposure of the permanent chondrocytes to Con A (5 micrograms/ml) for 24 hours and subsequent incubation in its absence for 5-10 days resulted in 10- to 100-fold increase in alkaline phosphatase and binding of 1.25 (OH)2 vitamin D3 to cells. Treatment with Con A also resulted in 10- to 20-fold increase in calcium content and 45Ca incorporation into insoluble material. Methyl-D-mannopyranoside reversed the effect of Con A on [35S]sulfate incorporation into proteoglycan and alkaline phosphatase activity. Since other lectins, such as wheat germ agglutinin, lentil lectin, phytohemagglutinin, Ulex europeasu agglutinin and garden pea lectin had been tested to have little effect on [35S]sulfate incorporation into proteoglycans and induction of alkaline phosphatase activity, the Con A action on chondrocytes seems specific. These results indicate that Con A is a potent modulator of differentiation of chondrocytes, which induces the onset on a maturing and a terminal differentiation in chondrocytes, leading to extensive calcification of the extracellular matrix.
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PMID:[Stimulation of maturing and terminal differentiation by concanavalin A in rabbit permanent chondrocyte cultures]. 787 71

To investigate the effect of rapid correction of chronic metabolic acidosis on circulating intact parathyroid hormone (I-PTH) activity by free calcium clamp in chronic renal failure, 18 patients were enrolled in this study. Metabolic acidosis was corrected by continuous bicarbonate infusion while plasma ionized calcium was clamped at the preinfusion level throughout the entire procedure. The plasma pH, bicarbonate, total CO2, sodium, serum total calcium and 1,25(OH)2 vitamin D3 levels increased significantly while serum concentrations of I-PTH, alkaline phosphatase and albumin showed significant decreases after bicarbonate infusion. The plasma ionized calcium, potassium, serum magnesium and inorganic phosphorus levels showed no significant difference before and after bicarbonate infusion. These results demonstrate that rapid correction of metabolic acidosis attenuates circulating PTH activity in chronic renal failure and may underline the importance of maintaining normal acid-base homeostasis particularly in the presence of secondary hyperparathyroidism.
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PMID:Rapid correction of metabolic acidosis in chronic renal failure: effect on parathyroid hormone activity. 796 74


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