Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

For the purpose of detecting the synthesis of zinc metalloenzymes after zinc supplementation in an experiment using rats the animals were first depleted for 15 days and subsequently injected a labelled zinc salt solution (65Zn-ZnCl2) in a dosis of 0.4 mg (in terms of Zn) and with an activity of 3.0 muCi/100microliter. After a 5-day depletion period, the activity of the metallo-enzymes alkaline phosphatase in plasma and in the femur and carboxy-peptidase A and B in the pancreatic gland was found to rise at the same rate as the 65Zn-measuring rate in plasma, femur and pancreatic gland. By calculating correlations this interdependence was demonstrated. Thus the highly significant correlation coefficients prove for these metalloenzymes that a synthesis with the injected zinc salt has taken place whilst for carbo-anhydrase in blood this evidence was not furnished. As the zinc dosis is not exclusively used for the enzyme synthesis, but additional 65Zn is incorporated into the individual organs, it does not appear to be possible to draw conclusions from the 65 Zn-measuring rate in the individual organs on the intermediary availability.
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PMID:[Demonstration of zinc-metalloenzyme syntheses after zinc supplements for deficient animals by means of measurement of the incorporation of 65Zn in various organs]. 41 27

In the livers of pregnant rats an anabolic effect was found for zinc which, however, developed analogously to the enlargement of the organ. Femur and muscle did not reveal any super-retention of zinc during pregnancy. In the whole organism, a 10% increased zinc storage was found being attributable to stronger accumulation in the reproductive organs (3%) and in the liver (1%) and in other organs (6%) that were not under study. Higher Zn supply did not allow to increase this additional zinc storage during pregnancy. After lactation, the anabolic effect in the livers had declined, the Zn values of the lactating rats remaining, on the whole, at the same level as those of the pregnant animals. The Zn-serum concentrations as well as the alkaline phosphatase and carboxy peptidase A activities of the pancreatic gland were reduced during pregnancy, whilst the alkaline phosphatase activity in the femur increased. This finding is discussed in relation to Zn-supply and Zn-mobilisation.
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PMID:[Zinc anabolism during pregnancy]. 67 11

A study was undertaken to assess the impact of the protein nature and soya antigenicity on the morphology and some enzyme activities of the jejunum in preruminant calves. Twenty Holstein calves fitted with a duodenal cannula were fed a liquid diet based on skimmed milk powder (SMP) for 2 weeks. They were then switched onto diets containing a mixture of SMP and either antigenic heated soybean flour (HSF; n = 12) or hypo-antigenic soya protein concentrate (SPC; n = 8) for 8 weeks, after which they were reverted back to the SMP diet for 2 weeks. The diets contained similar amounts of digestible nitrogen and energy, and were fed at a rate of 55 g DM/kg(0.75)/d. Proximal jejunal biopsies were collected just before (week 0), during (weeks 2 and 8) and after (week 10) feeding of the soya-based diets, and were used for morphology measurements and the determination of total alkaline phosphatase, lactase, amino-peptidases A and N, and dipeptidyl peptidase IV activities. Feed intake and growth were similar between the HSF and SPC groups during the experimental period. The effects of antigenicity and the antigenicity x time interaction were never significant (P > 0.05). Villus height decreased (P < 0.01) between weeks 0 and 2, and increased (P < 0.05) between weeks 8 and 10. Villus width increased between weeks 2 and 8 (P < 0.001). Crypt depth also increased between weeks 0 and 2 (P < 0.001). Specific activities of alkaline phosphatase (P < 0.01) and amino-peptidase N (P < 0.05) decreased between weeks 0 and 2. Conversely, those of alkaline phosphatase (P < 0.0001), lactase (P < 0.01) and dipeptidyl-peptidase IV (P < 0.0001) increased between weeks 8 and 10. Specific activities for lactase and amino-peptidase N decreased (P < 0.01) between weeks 2 and 8. The treatments had little effects on the amino-peptidase A activity. In conclusion, the present work demonstrated that soybean protein markedly depressed the morphology and most enzyme activities of the calf small intestine. On the contrary, the in vitro antigenicity of soybean protein had little influence on these parameters in this study.
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PMID:Morphology and enzyme activities of the small intestine are modulated by dietary protein source in the preruminant calf. 1049 51