Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cytochemical tuberculin-associated shifts along with their prognostic evaluation were studied in 30 patients with silicosis and silicotuberculosis with intrathoracic adenopathy. The study revealed that a decrease of alkaline phosphatase activity of neutrophils, alpha-glycerophosphate dehydrogenase and succinate dehydrogenase lymphocytes could serve as an early indicator of tuberculosis process in the pulmonary tissue and intrathoracic lymph nodes.
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PMID:[Tuberculin-cytochemical test in the differential diagnosis of silicosis and silicotuberculosis with intrathoracic adenopathy]. 270 4

Enzyme histochemical techniques were utilized to examine the progression and extent of proximal tubular injury during the development of cis-diamminedichloroplatinum (II) (CDDP)-induced acute renal failure. Acute renal failure was induced in male rats by the intraperitoneal administration of 10 mg CDDP/kg body weight. At 6, 24, 48, 72, and 96 hr following treatment, renal function was assessed and tissue was collected for renal morphologic and enzyme histochemical studies. The enzymes examined were gamma-glutamyl transpeptidase, alkaline phosphatase, sodium-potassium ATPase (nitrophenyl phosphatase), acid phosphatase, glucose-6-phosphatase, succinic dehydrogenase, alpha-glycerophosphate dehydrogenase, and lactic dehydrogenase. By 24 hr, the activity of acid phosphatase was reduced throughout the proximal tubule, with the greatest decrease occurring in the P3 segment of the proximal tubule located in the outer stripe of the outer medulla. Changes in the histochemical staining of the remaining enzymes were not consistently observed until 48 or, in some cases, 72 hr. These alterations involved all portions of the proximal tubule with the most severe changes involving P3. The results of the enzyme histochemical studies along with the morphologic findings indicating that the initiation of CDDP-induced acute renal failure, first apparent at 48 hr in this model, is associated with cell injury throughout the proximal tubule. The majority of the histochemical changes did not become apparent until late in the course of tubular injury. This suggests that most of the changes in enzyme activity represent nonspecific effects of CDDP-induced tubular injury, as opposed to direct enzyme inhibition by the drug.
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PMID:Cis-diamminedichloroplatinum (II)-induced acute renal failure in the rat: enzyme histochemical studies. 287 24

The rate of perinatal encephalopathy and the nature of its outcomes in relation to the syndrome of the acute period of the disease were studied in 102 children. Also examined were the cytochemical parameters of blood leukocytes, alkaline phosphatase of neutrophils, acid phosphatase of lymphocytes, succinate and alpha-glycerophosphate dehydrogenase of lymphocytes in prematurely born infants on the 8th-15th and the 30th-45th days of life. It has been ascertained that in addition to the comatose and convulsive syndromes the prognosis of the disease is the least favourable in the syndrome of total inhibition of the CNS in the acute period. Furthermore, the findings of the conducted study open the possibility of preliminary individual prognosis of the disease on the basis of the cytochemical picture at the end of the acute period.
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PMID:[Development of children with a history of perinatal encephalopathy]. 342 43

Activity of some enzymes in the cerebral cortex of rats with experimentally induced renal arterial hypertension (AH) was studied histochemically. Histologic data provide the evidence for the disturbances in water-salt metabolism in AH. Morphometric study revealed an increase in the specific volume of smaller microvessels and moderate decrease in the specific volume of bigger microvessels. Vascular markers show different time course of activity changes in AH: the activity of alkaline phosphatase increases, while that of alpha-glycerophosphate dehydrogenase remains unchanged. The development of AH is accompanied by the increase in succinic dehydrogenase activity and the decrease in the activity of lactate dehydrogenase in neurons. The changes in the neuron-capillary relationship arising in AH can be one of the possible pathogenetic factors in the pathologic process progression.
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PMID:[Characteristics of the morphofunctional status of the tissue in the rat cerebral cortex in experimental renal arterial hypertension]. 366 63

We describe methods for automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid. Phospholipase C (EC 3.1.4.3) and sphingomyelin phosphodiesterase (EC 3.1.4.12) are reacted with lecithin and sphingomyelin, respectively, to liberate phosphocholine. Phosphocholine is then reacted with alkaline phosphatase, choline oxidase, peroxidase, and 4-aminoantipyrine to form a colored complex, for which the absorbance at 500 nm is measured with a centrifugal analyzer. Phosphatidylglycerol is hydrolyzed by phospholipase D (EC 3.1.4.4) to form glycerol, which is subsequently reacted with ATP and NAD+ in the presence of glycerol kinase and glycerol-3-phosphate dehydrogenase to yield NADH. The absorbance of the NADH formed is measured at 340 nm. These methods provide a simple, rapid, and accurate alternative to thin-layer chromatography for determination of phospholipids in amniotic fluid for assessment of fetal lung maturity.
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PMID:Automated enzymatic measurement of lecithin, sphingomyelin, and phosphatidylglycerol in amniotic fluid. 380 1

Cytochemical methods were used to explore the changes in the activity of redox (succinate dehydrogenase, mitochondrial alpha-glycerophosphate dehydrogenase) and hydrolytic (acid, alkaline phosphatase, myeloperoxidase) enzymes of peripheral blood leukocytes of random-bred albino rats during simulation of toxic hepato-, nephropathy and hemic hypoxia of different gravity. The studies of the changes of leukocyte distribution according to the activity of the above enzymes were demonstrated to be informative for the diagnosis of prepathological conditions. The possibility was also shown of differentiating the gravity of the pathological conditions with the aid of an analysis of the enzymes under study by the method of linear discriminant functions.
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PMID:[Information value of research on the enzyme status of the peripheral blood leukocytes in a toxicological experiment]. 397 44

Hypercholesterolemia, produced in rabbits after long-term feeding with cholesterol, was accompanied by an increase in activity of alkaline phosphatase and LDH in blood serum as well as by a decrease in activity of intracellular dehydrogenases of lymphocytes. After hemosorption content of cholesterol and activity of blood serum enzymes were decreased; activity of alpha-glycerophosphate dehydrogenase tended to decrease.
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PMID:[Determination of the serum enzyme and dehydrogenase activity of circulating lymphocytes during hemosorption in dietary hypercholesterolemia]. 400 56

Relapsing fever borreliae require lipid compounds for growth in vitro. In this study, the major pathways of lipid catabolism in three species of tick-borne relapsing fever borreliae were investigated. Thin-layer chromatography was used to compare chloroform-methanol extracts of fresh culture media with extracts of exhausted culture media after organisms were removed by centrifugation. The chromatographic data demonstrated that lysolecithin was removed from the culture media during growth of the spirochetes, whereas lecithin, sphingomyelin, triglycerides, and cholesterol esters were not affected by growth of the organisms. Sonic extracts of the organism were tested for the presence of specific enzymes of lipid catabolism. Lysolecithinase, glycerophosphorylcholine diesterase, and acid phosphatase activities were demonstrated. Thus, these organisms can sequentially dissimilate lysolecithin to fatty acids, choline, inorganic phosphate, and glycerol. Assays for phospholipases A, C, and D, alpha-glycerophosphate dehydrogenase, alkaline phosphatase, and lipase were negative.
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PMID:Lipid catabolism of relapsing fever borreliae. 436 Dec 92

Natural populations of Glossina morsitans submorsitans Newstead, Glossina palpalis gambiensis Vanderplank and Glossina tachinoides Westwood occurring within 150 km of Bobo-Dioulasso, Upper Volta were examined using polyacrylamide gel electrophoresis. No variation was found in the banding pattern for arginine phosphokinase (EC 2.7.3.3). G. p. gambiensis and G. tachinoides had three alleles for each of the thoracic enzymes octanol dehydrogenase (EC 1.1.1.73), malic dehydrogenase (EC 1.1.1.37) and alpha-glycerophosphate dehydrogenase (EC 1.1.1.8) and for midgut alkaline phosphatase (EC 3.1.3.1). The same situation was found with G. m. submorsitans except that only two alleles for alpha-glycerophosphate dehydrogenase and one for alkaline phosphatase were found. In each species, and for each of the enzymes, one allele was present at a frequency of 95% or greater. There was little or no variation between populations. Two laboratory colonies of G. p. gambiensis had less genetic variation than wild populations.
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PMID:Genetic polymorphism in three species of tsetse flies (Diptera: Glossinidae) in Upper Volta. 611 54

Isozyme profiles for 32 enzyme systems were studied in tumors induced by two strains of polyoma virus (2PTA and LID1), in two conventional mouse strains (C3H/BiDa and NIH), and in athymic (nude) mice of two genetic backgrounds (C3H/Hes nu/nu and NIH nu/nu). Tumors studied were: primary and transplant passages of salivary gland tumors (127); primary thymic epithelial tumors (12); primary subcutaneous sarcomas (6); primary hair follicle tumors (5); primary and transplant passages of mammary tumors (18); primary ameloblastomas (3); and primary renal medullary sarcomas (3). Regardless of mouse strain or virus strain, the isozyme arrays were highly constant and unique for each tumor histotype with the exception of salivary and mammary tumors, which shared a single profile differing from that of each of the other histotype-associated profiles. Other tumor types could be distinguished from each other and from the salivary-mammary tumor pair by as few as five isozymes: glycerol-3-phosphate dehydrogenase; glyceraldehydephosphate dehydrogenase; lactate dehydrogenase; sorbitol dehydrogenase; and alkaline phosphatase. Twelve nonpolyoma mammary tumors and their passages from mouse mammary tumor virus-expressed C3H/Hes nu/+ mice were analyzed for the same enzymes; variations in activity and isozyme profiles were found for ten enzyme systems. Three spontaneous salivary myoepitheliomas in BALB/c mice were also analyzed; two different lactate dehydrogenase profiles were observed, and all three tumors lacked the placental alkaline phosphatase present in polyoma virus-induced salivary tumors. Uniformity of isozyme phenotype may be characteristic of DNA virus transformation of cells in a particular differentiative state. This uniformity does not appear to occur in mouse mammary tumor virus-associated tumors, spontaneous tumors, and, according to the literature, chemically induced tumors.
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PMID:Isozyme phenotypes of polyoma virus tumors in mice. 630 95


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