Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.3.1 (
alkaline phosphatase
)
47,916
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Aflatoxin B1 (AFB1) pollution is one of the most serious problems for food safety. In this paper, a split-type photoelectrochemical (PEC) immunoassay was designed for sensitive detection of AFB1 in foodstuffs by using amorphous
TiO2
with all-inorganic perovskite CsPbBr3 nanocrystals (CsPbBr3/a-
TiO2
). The a-
TiO2
layer not only improved the stability of CsPbBr3 nanocrystals, but also facilitated charge transfer, which resulted in the increasing photocurrent of the nanocomposites. Initially, a competitive-type enzyme immunoreaction was executed on a high-binding microplate between the analyte and
alkaline phosphatase
(
ALP
)-labeled AFB1-bovine serum albumin (AFB1-BSA) conjugate. Accompanied by the formation of the immunocomplex, the carried
ALP
triggered enzymatic hydrolysis to generate ascorbic acid (AA, as an electron donor) for increasing the photocurrent of the CsPbBr3/a-
TiO2
-modified electrode. Coupling with the competitive enzyme immunoassay, the photocurrent of the modified electrode decreased with the increase of target AFB1 concentration in a dynamic working range from 0.01 ng mL-1 to 15 ng mL-1 with a limit of detection (LOD) of 2.8 pg mL-1 under optimum conditions. Furthermore, the photoelectrochemical immunoassay was also utilized to detect AFB1 in peanut and corn samples, giving acceptable accuracy in comparison with the referenced AFB1 enzyme-linked immunosorbent assay (ELISA) method.
...
PMID:Photoelectrochemical immunoassay of aflatoxin B
1
in foodstuff based on amorphous TiO
2
and CsPbBr
3
perovskite nanocrystals. 3129 69
Membranes which have an osseointegration abilty are often selected as biomaterials in oral and maxillofacial surgery. Although these membranes are often the best option for certain uses, it is a challenge to create functionally attractive membranes. In this research, electro-spun titanium oxide (
TiO2
)/hydroxyapatite (HA)/polyurethane (PU) membranes were fabricated with different ratios of HA and
TiO2
: 100: 0, 70:30, 50:50, 30:70 and 0:100 w/w. The morphologies of the different mixtures were assessed with a Scanning Electron Microscope (SEM) and Field Emission Microscope (FESEM). Element analysis was performed with EDX. The physical properties of the water contact angles and mechanical strength were tested and the membranes cultured with osteoblasts to evaluate their biological functions, cell adhesion, viability, proliferation,
alkaline phosphatase
(
ALP
) activity, and calcium content. The results showed that the membranes with TiO
2
and HA had smaller fibers than those without TiO
2
and HA. The
TiO2
- and HA-including compounds showed the formation of particle aggregation on the surface of the fibers. They also had higher water contact angles, mechanical strength, and stiffness than those without TiO
2
and HA, and they had better cell adhesion, viability, proliferation,
ALP
activity and calcium content. The membrane with a 50:50 TiO
2
:HA ratio had more unique biological functions than the others. Finally, our research demonstrated that osseointegrated membranes with 50:50
TiO2
:HA are promising for oral and maxillofacial surgery.
...
PMID:Osseointegrated membranes based on electro-spun TiO
2
/hydroxyapatite/polyurethane for oral maxillofacial surgery. 3192 63
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