Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Three marker enzymes were measured during treatment of psoriatic plaques with two different therapies. During treatment with clobetasol propionate the epidermal enzymes (acid phosphatase and glucose-6-phosphate dehydrogenase) returned to normal within 14 days whereas capillary alkaline phosphatase remained at the original level. By contrast, all three marker enzymes reverted to normal at the same tempo during PUVA therapy, reaching the control range after 4-8 weeks.
Br J Dermatol 1984 Apr
PMID:Metabolic changes in the psoriatic lesion during therapy. 671 85

Plasma zinc, serum albumin and alkaline phosphatase (a zinc-dependent enzyme) were measured in thirty-five psoriatics and their age- and sex-matched controls. No significant difference was seen between these two groups as a whole, but psoriatics with less than 10% surface involvement had significantly higher mean plasma zinc levels than their respective control group. Psoriatic patients also showed a relationship between the extent of surface involvement and the plasma zinc level, those with more extensive involvement having lower levels than those with minimal involvement. The differences were not due to disturbances in serum albumin, and no abnormalities in serum alkaline phosphatase were observed.
Br J Dermatol 1983 Mar
PMID:Plasma zinc in psoriasis: relation to surface area involvement. 683 Jun 95

Keratotome slices were cut across the margins of rapidly-spreading psoriatic plaques. Each slice was divided into eight sections and in each section we measured the percentage cells in S phase and the levels of glucose-6-phosphate dehydrogenase (both related to epidermal proliferation), acid phosphatase (associated with keratinization) and alkaline phosphatase (a marker for dermal capillaries). Disturbances in the epidermis extended only 2 to 4 mm into the 'uninvolved' skin, whereas the capillaries were metabolically abnormal for a distance of about 2 cm ahead of the advancing edge of the plaque. This implies that changes in the capillary may precede those in the epidermis during the spread of the psoriatic lesion.
Br J Dermatol 1983 Jun
PMID:Metabolic changes at the margin of the spreading psoriatic lesion. 686 May 60

A new immunoperoxidase staining technique of high specificity (the labelled antigen method) has been used for the detection of serum proteins in the epithelium of human skin and mucous membranes. The majority of specimens contain cells showing strong diffuse intracellular staining. These cells usually lie in the upper mid-zone of the epithelium and are often characterized by vacuolated or pyknotic nuclei. Double immunoenzymatic labelling (using alkaline phosphatase and peroxidase) showed that the different proteins are usually present in the same cells. A less frequent pattern of staining took the form of delicate strands of intercellular IgG. Samples from a wide range of skin diseases were studied, but no clear relationship between positive epithelial staining and disease states was apparent. It is suggested that serum proteins may enter epithelial cells by passive diffusion, and attention is drawn to other instances (e.g. in the central nervous system and lymphoid tissue) where similar phenomena have been observed by immunoenzymatic staining techniques.
Br J Dermatol 1980 May
PMID:Serum proteins in human squamous epithelium. 699 42

Dendritic alkaline phosphatase-positive cells in the epidermis of cattle were studied. By light microscopy they were shown to occur in basal and suprabasal positions in the epidermis, with their dendrites reaching into outer layers of the stratum spinosum. They were also found in the external root sheaths of hair follicles. In separated epidermal sheets from ears of cattle these cells were found to be uniformly distributed with an average concentration of approximately 1,600 cells/mm2. Langerhans cells were identified in the epidermis by the presence of dendritic cells of Langerhans cell granules, a lobated nucleus and clear cytoplasm and the absence of desmosomes, tonofilaments, premelanosomes, and melanosomes. Alkaline phosphatase activity was associated with the plasmalemma of such cells and occasionally with cytoplasmic inclusions which may have been Langerhans cell granules. Enzyme activity was not associated with melanocytes or keratinocytes. It is confirmed that alkaline phosphatase activity is a feature of Langerhans cells, and possibly "indeterminate" cells in the normal epidermis of cattle.
J Invest Dermatol 1982 Jul
PMID:Alkaline phosphatase-positive Langerhans cells in the epidermis of cattle. 708 75

A fluorometric microassay of alkaline phosphatase is described; it is based on the hydrolysis of 4-methylumbelliferyl phosphate and is about three orders of magnitude more sensitive than the usual colorimetric procedures. When applied to skin, the addition of fluoride is necessary to avoid interference from the relatively high levels of acid phosphatase present in the epidermis. Kinetic data for cutaneous alkaline phosphatase are reported. Skin appears to contain a single enzyme of the 'bone-liver-kidney' type, which is present both in a soluble and in membrane-bound form. It occurs almost exclusively in the dermis, not more than 1% of the total alkaline phosphatase of human skin being present in the epidermis.
Arch Dermatol Res 1982
PMID:Cutaneous alkaline phosphatase: a biochemical study. 716 73

A case report is presented of a young woman in whom symptomatic porphyria cutanea tarda (PCT) developed during copper chelation therapy for Wilson's disease. The 22 year old white woman was seen in the summer of 1978 because of development of blisters on the dorsa of the hands associated with focal atrophic hypopigmentation, generalized hyperpigmentation of the skin, and hpertrichosis of the lateral forehead and face. A sibling had died in childhood with Wilson's disease. When the patient developed hepatomegaly, ascites, and an acute hepatitis syndrome at the age of 11, penicillamine therapy was empirically started, with gradual symptomatic improvement. When evaluated at the age of 22, abnormal laboratory values included a total bilirubin of 1.2 mg%; alkaline phosphatase, 96 U; serum glutamic oxaloacetic transaminase (SGOT), 175 U; serum glutamic pyruvic transaminase (SGPT), 122 U; gamma glutamyl trans peptidase (GGTP), 64 U; and Bromsulphalein (BSP) retention, 21% at 45 minutes. Skin biopsy from the hand revealed a noninflammatory subepidermal bulla with prominently PAS positive vessel walls in the festooned dermal papillae at the base of the blister. A fragmented liver biopsy failed to reveal evidence of active hepatitis or cirrhosis, but considerable stainable iron was present in both hepatocytes and Kupffer cells. A rubeanic acid stain for copper was negative. The patient was diagnosed as having Wilson's disease, hepatic hemosiderosis, and PCT. Cessation of all ethanol consumption and discontinuation of the oral contraceptives which she had been taking for 6 years, was recommended. On examination 9 and 22 months after these modifications were instituted, the patient felt asymptomatic and was without evidence of any new blisters or scars of her skin. The hyperpigmentation and hypertrichosis persisted, but she rigidly adhered to a program of penicillamine, topical sunscreen application, and abnegation of alcohol. Liver function studies were normal, and urinary porphyrin levels returned toward normal values. The clinical onset of this patient's blistering disease was temporally associated with ethanol and exogenous estrogen medication.
J Am Acad Dermatol 1981 Jan
PMID:Porphyria cutanea tarda complicating Wilson's disease. 720 91

A unique cas of dermal calcification occurred in a 14-month-old femal infant. Results of all pertinent laboratory data obtained on this patient were within normal limits except for an elevation of the heat labile fraction of alkaline phosphatase. A skin biopsy specimen showed irregularly shaped deposits of calcified osteoid with osteoblasts. As yet, neither the cause nor the prognosis of this disease has been delineated.
Arch Dermatol 1981 Dec
PMID:Dysplastic cutaneous osteomatosis. A unique case of true osteoma. 731 57

A 38-year-old black woman presented with a multisystem disease characterized by malaise, fever, sweats, and diffuse hyperpigmentation. Laboratory examinations showed anemia, elevated alkaline phosphatase, granulomas in the liver and bone marrow, and elevated porphyrins in the urine and feces, characteristic of porphyria cutanea tarda (PCT). A workup for an etiology of the granulomas was nonproductive, and the patient responded to systemic steroids.
J Am Acad Dermatol 1980 Jan
PMID:Porphyria cutanea tarda and sarcoidosis. 735 54

The activity of alkaline phosphatase (AAP) in papillary capillaries of normal and psoriatic skin was characterized by enzyme inhibition studies. Quantitatively, there were pronounced differences between normal and psoriatic skin, i.e., increase of AAP, as determined by a grading system, and assimilation of the strength of AAP in venous and arterial side of the capillary loop in psoriasis. Qualitatively, the inhibition studies with different actin inhibitors revealed no difference between AAP in normal and psoriatic skin or between initial, fully developed and healed psoriatic lesions as well as noninvolved skin of psoriatics. Thus, the physiologic AAP seems to be stimulated in psoriasis. Generally, AAP of dermal capillaries is highly sensitive to cysteine inhibition.
Arch Dermatol Res 1980
PMID:Characterization of activity of alkaline phosphatase (AAP) in capillary endothelium of normal and psoriatic skin. 740 28


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