Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Ornithine decarboxylase (ODC) of Tetrahymena pyriformis is inactivated by E. coli or calf intestinal alkaline phosphatase. Inactivated ODC by E. coli alkaline phosphatase, form b, can be allosterically reactivated to form a by purine-nucleosides or -nucleotides at 10(-4) M concentration. Inactivated ODC by calf intestinal alkaline phosphatase bound to agarose can be converted to form a by purine-analogues with 10(-7) M concentration only if inorganic phosphate is included in the incubation mixture. Pyridoxal phosphate, phosphoamino acids or other phospho-compounds are incapable to promote the conversion of b form to a form of ODC. These data suggest that purine-analogues may be the in vivo physiological regulators of ODC of T. pyriformis.
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PMID:Allosteric activation by purine nucleosides and nucleotides of the inactive by phosphatase ornithine decarboxylase of Tetrahymena pyriformis. 313 58

We studied the effects of phosphates on the expression of the human tissue-nonspecific alkaline phosphatase (TNSALP) gene and phosphate-regulating genes in short-term cultures of human osteoblastic osteosarcoma cell lines. When human osteosarcoma cell lines, SaOS-2, MG-63, and U(2)OS were cultured with 10 mM inorganic sodium dihydrogenphosphate, 10 mM beta-glycerophosphate, 250 microM pyridoxal phosphate, or 100 microM inorganic pyrophosphate, enzymatic activity of alkaline phosphatase began to increase at 72 h after addition of sodium dihydrogenphosphate and beta-glycerophosphate in SaOS-2 cells. Pyridoxal phosphate and pyrophosphate did not induce alkaline phosphatase activity. U(2)OS cells slightly reacted to beta-glycerophosphate, but MG-63 cells did not react on exposure to phosphates. In SaOS-2 cells, TNSALP mRNA measured by real-time RT-PCR reached a peak level at 72 h after the addition of beta-glycerophosphate. PHEX and MEPE mRNAs were also induced by beta-glycerophosphate. These results suggest that TNSALP, PHEX and MEPE were concordantly induced by beta-glycerophosphate on mineralisation.
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PMID:Effects of phosphates on the expression of tissue-nonspecific alkaline phosphatase gene and phosphate-regulating genes in short-term cultures of human osteosarcoma cell lines. 1631 17