Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Acyl-CoA hydrolase activities, using palmitoyl-CoA and decanoyl-CoA as substrates, were highest in the proximal part and lowest in the distal part of the guinea-pig small intestine. Butyryl-CoA hydrolase activity was not found in any of the homogenates. 2. The acyl-CoA hydrolases showed a complex subcellular distribution when compared to classical marker enzymes. The specific activity of the hydrolase was highest in the microsomal fraction, and lowest in the soluble fraction when palmitoyl-CoA was used as substrate. When decanoyl-CoA was used as substrate, highest activity was found in the mitochondrial/lysosomal fraction and lowest in the microsomal fraction. 3. Gel filtration on an ultrogel AcA-44 column separated the palmitoyl-CoA hydrolase of the cytosol fraction into three or four fractions. 4. Palmitoyl-carnitine hydrolase was present in the microsomal and the nuclei fractions. The distribution was mostly similar to the alkaline phosphatase suggesting a brush border localization.
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PMID:A study of the distribution of acyl-CoA hydrolases and acyl-L-carnitine hydrolases in guinea-pig small intestine. 612 4

ATPase of 14S dynein, extracted from spermatozoa of the sea urchin, Hemicentrotus pulcherrimus, and partially purified by sucrose density gradient centrifugation, was inhibited non-competitively by palmitoyl CoA at concentrations higher than 20 microns, and was stimulated at concentrations between 2 microns and 10 microns. The effects of palmitoyl CoA on dynein ATPase were reversed by bovine serum albumin (1 mg/ml) and spermine (0.1 and 1 mM). Myristoyl CoA exerted effects similar to those of palmitoyl CoA. Short chain fatty acyl CoAs, such as butyryl CoA, propionyl CoA and acetyl CoA, CoA, Na-palmitate, Na-myristate, and palmitoyl carnitine had hardly any effect on dynein ATPase. Palmitoyl CoA failed to inhibit purified CF1 ATPase from chloroplasts of spinach, ATPase of rat liver mitochondria and alkaline phosphatase from calf intestine.
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PMID:Inhibition by palmitoyl CoA of dynein ATPase from sea urchin spermatozoa. 621