Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of Misoprostol (0.3 mg/kg b.w., orally for four weeks) on the brush border membrane enzyme activity, is studied in growing rats. Misoprostol enhanced stomach and intestine relative weights as well as the mucosal weight of the duodenum and proximal jejunum. In treated rats, disaccharidases, alkaline phosphatase and aminopeptidase enzyme activity were measured in brush border purified fraction throughout the small intestine. Sucrase, maltase, aminopeptidase and alkaline phosphatase specific activities were significantly increased along the small intestine. In the proximal jejunum, sucrase (62%; p < 0.001) and maltase (42%; p < 0.01) activities were significantly greater. Sucrase activity was also significantly (p < 0.001) increased by about 103% in the distal jejunum. There was also a significant (p < 0.05) increment of 32% in the duodenal and ileal alkaline phosphatase activity after treatment. Similarly, aminopeptidase activity was significantly (p < 0.05) higher in duodenum (67%) and jejunum (24%). In conclusion, Misoprostol appreciably increased the ability of the small intestine to perform its digestive functions although further studies will be necessary to examine the cellular and molecular mechanism(s) which may be responsible for these effects.
 ...
PMID:Stimulation of brush border enzyme activity along the rat small intestine by misoprostol. 780 Sep 17

The aim of this study was to investigate the effect of misoprostol, silymarin or the co-administration of misoprostol + silymarin on the carbon tetrachloride (CCl(4))-induced hepatic injury in rats. Misoprostol (10, 100, 1000 microg/kg), silymarin (25 mg/kg) or misoprostol (100 microg/kg) + silymarin (25 mg/kg) was given once daily orally simultaneously with CCl(4) and for 15 days thereafter. The results showed that misoprostol (10, 100 or 1000 microg/kg) conferred significant protection against the hepatotoxic actions of CCl(4) in rats, reducing serum alanine aminotransferase (ALT) levels by 24.7%, 42.6% and 49.4%, respectively compared with controls. Misoprostol, given at 100 or 1000 microg/kg, decreased aspartate aminotransferase (AST) by 28 and 43.6% and alkaline phosphatase (ALP) by 19.3% and 53.4% respectively. Meanwhile, silymarin reduced ALT, AST and ALP levels by 62.7%, 66.1% and 65.1% respectively. The co-administration of misoprostol (100 microg/kg) and silymarin (25 mg/kg) resulted in 61.4%, 66.1% and 57.5% reduction in ALT, AST and ALP levels respectively. Histopathological alterations and depletion of hepatocyte glycogen and DNA content by CCl(4) were markedly reduced after treatment with misoprostol, silymarin or misoprostol + silymarin. Image analysis of liver specimens revealed a marked reduction in liver necrosis; area of damage: 32.4%, 24% and 10.2% after misoprostol (10, 100 or 1000 microg/kg), 7.2% after silymarin and 10.9% after treatment with misoprostol 100 microg/kg + silymarin, compared with CCl(4) control group (46.7%). These results indicate that treatment with misoprostol protects against hepatocellular necrosis induced by CCl(4). This study suggests a potential therapeutic use for misoprostol in liver injury.
 ...
PMID:Hepatoprotective effects of misoprostol and silymarin on carbon tetrachloride-induced hepatic damage in rats. 1929 38