Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spontaneously hypertensive rats (SHR) and two strains of normotensive rats were compared with respect to enzymatic activities and calcium accumulation of plasma membrane and endoplasmic reticulum enriched fractions from their mesenteric arteries. Increased specific activities of alkaline phosphatase, 5'-nucleotidase and Mg2+-ATPase, and increased ATP-dependent calcium accumulation were found in 5- to 6-month-old SHR as compared to both strains fo age-matched normotensive rats. Alkaline phosphatase was increased in 33-day-old "early hypertensive" and 3- to 4-month-old SHR, but 5'-nucleotidase, Mg2+-ATPase, and calcium accumulation were not. Hydralazine treatment of young SHR partially prevented the increase of both alkaline phosphatase activity and blood pressure that develops with age. The relationship between alkaline phosphatase activity and the alterations in vascular reactivity associated with hypertension remains to be determined.
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PMID:Relationship between blood pressure of spontaneously hypertensive rats and alterations in membrane properties of mesenteric arteries. 13 88

Human adult lung fragments removed from macroscopically undamaged and anthracosis exempted zones of lungs of 20 pneumonectomies made for cancer, were tested for 25 enzymic activities. The location and intensities of these enzymic activities were different in the lung tissue components; The bronchial epithelia contained highly active LDH, MDH, SDH, NADH-TR and NADPH-TR, glucose-6-phosphate dehydrogenase, active hydroxyproline-2-epimerase, alkaline phosphatase. Ca2+-activated ATP-ase, and beta-galactosidase. Bronchial and vascular muscles presented intense activities of LDH, MDH and SDH of alkalinephosphatase, AMP-ase and Ca2+-activated ATP-ase, as well as of beta-galactosidase. The alveolar walls presented high activities of SDH, MDH and LDH, of alkaline and acid phosphatases, of beta-galactosidase and of Tween-40 and 60-esterases, of HEP, cytochrome-oxidase and peroxidase. The free alveolar macrophages were active for LDH, MDH, SDH, NADH-TR and NADPH-TR, G1-6-ph-DH, acid and alkaline phosphatase, cytochrome-oxidase and peroxidase, HEP, AMP-ase and Mg2+-activated ATP-ase, Tween-esterases, naphthol-ASD-acetate esterase, and beta-galactosidase. The endothelia contained high activities of alkaline phosphatase, of AMP-ase and Mg2+-activated ATPase, of LDH, MDH and SDH, and of beta-galactosidase. In bronchial lymphoid nodules it was the LDH, MDH, SDH, cytochrome-oxidase and peroxidase, HEP, alkaline phosphatase and AMP-ase, Tween-60-esterase and beta-galactosidase that were active. The interlobular areas of the lung presented intense activities of SDH, MDH, LDH, HEP and cytochrome-oxidase. The activities of the other tested enzymes were weaker or absent in the adult human lung components, the same as those of aminopeptidases which were present only in some free alveolar macrophages. The discussion of some relationships between these enzymic actitivies and the morphology of the human adult lung tissue asserted that the latter could not be considered as a "normal" tissue but as one overstrained by the components of blood and polluted air.
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PMID:Histoenzymology of the lung. I. Enzyme activities of the lung tissue of acult humans; relationships between structure and functions. 14 Mar 14

In the present investigation the results of a lead salt technique and two calcium salt techniques for the deomonstration of the activity of myosin adenosine triphosphatase in sections of both normal and pathological human skeletal muscle specimens are compared. It was seen that the histochemical results obtained by the different techniques are similar, especially with regard to the identification of fibre-types. It can be clearly stated, that the alkaline phosphatase activity present in muscle fibers of diseased skeletal msucles revealed only a very slight activity with the substrate ATP, so the alkaline phosphatase activity in general did not disturb the reliability of the different myosin ATPase techniques. Moreover it was found that the presence of the mitochondrial Ca2+ -ion activated ATPase with a high pH-optimum in muscle fibers did not give rise to faulty results. From studies with dinitrophenol it can be concluded that this substance activates the myosin ATPase present in type I fibres especially.
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PMID:The value of enzyme histochemical techniques in the classification of fibre types of human skeletal muscle. 2. The histochemical demonstration of myosin adenosine triphosphatase in skeletal muscles from adult patients with or with no diseases of the neuromuscular system. A comparison between results obtained by calcium salt and lead salt techniques. 14 Aug 52

Novoimanine is an antibacterial drug from Hypericum perforatum L. When used in the bacteriostatic concentration, i.e. 0.5 gamma/ml, it induced release of potassium ions from the cells of Staphylococcus aureus 209P and had no effect on release of the UV-absorbing compounds and 14C-amino acids. In addition, incubation of the cells with novoimanine (2.5--50 gamma/ml) provided "preservation" in them of the earlier absorbed 14C-amino acids, while in the control cells their level decreased. In a concentration of 100 gamma/ml novoimanine stimulated activity of ATP-ase and alkaline phosphatase by 34 and 37-57 per cent respectively. Histones F1 and F3 of the calf thymus induced an intensive release of 14C-amino acids from the cells of staphylococci and increased the activity of ATP-ase by 6-10 times. The data of the study suggested that the effect of novoimanine on the cytoplasmic membrane was limited and different from that on the polycationic antibacterial agents.
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PMID:[Effect of novoimanine on the cellular permeability indices of staphylococci]. 14 41

Mg-dependent ATP-ase, alkaline phosphatase and carbonic anhydrase activities were tested at the level of capillary endothelia of alveolar walls in normal rabbits. A constant and relatively intense activity within these endothelia appeared to be that of the Mg-dependent ATP-ase. The carbonic anhydrase activity marked some endothelia, and the alkaline phosphatase was unequally demonstrable at this level. These different histoenzymic aspects might reflect the unequal activity during time and space of these enzymes at the level of capillary endothelia of alveolar walls.
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PMID:Histoenzymology of the lung. Some enzymic activities of capillary endothelia of the rabbit alveolar walls. 14 9

A simple and reliable technique for the visualization of capillaries in skeletal muscel of dogs, guinea pigs and rats is decribed. 10--20 micrometer frozen sections were incubated in a medium containing Ca2+ and ATP following acid preincubation (pH 3.8--4.2). The capillaries stained in black and were readily seen surrounding the muscle fibers. Serial sections were also treated for alkaline phosphatase. Values for capillary density using both methods were not different.
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PMID:Visualization of capillaries in skeletal muscle by the ATPase reaction. 14 63

1. Pretreatment of frozon cryostat sections with formaldehyde or calcium ions inhibits diffusion of the plasma membrane enzymes 5' nucleotidase, ATP-ase and alkaline phosphatase during incubation. 2. Treatment of fixed sections with different kinds of buffer at 37 degrees C induces diffusion of enzyme activity from the plasma membrane to other sites of the section and into the incubation medium. This buffer influence depends on temperature: at 4 degrees C only a slight diffusion occurs. Addition of phospholipase C, digitonin or taurocholate to the buffer opposes the buffer effect. 3. Pretreatment of frozen cryostat sections with a mixture of equal parts of chloroform and acetone give a good fixation of the plasma membrane enzymes 5'-nucleotidase, ATP-ase, alkaline phosphate and leucyl-beta-naphthylamidase. During this treatment the different kinds of lipids present in the membrane are ex-racted equally. After this fixation buffer treatment does not cause a visible diffusion of enzyme activity in the section. Only a slight diffusion (1 till 7 percent) into the buffer solution takes place. 4. The mentioned treatments open up possibilities to get insight into the membrane anchorage of plasma membrane enzymes.
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PMID:Influence of fixation and buffer treatment on the release of enzymes from the plasma membrane. 14 99

Rats were fed a low calcium diet deficient in vitamin D for 14 days. Changes in alkaline phosphatase activities in odontoblasts dissected out from incisor teeth were studied biochemically. A strong increase in pNPP-ase, PPi-ase, total ATP-degradation and Ca2+- ATPase was observed in the deficient animals compared with animals fed a control diet.
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PMID:Odontoblast metabolism in rats deficient in vitamin D and calcium. II. Changes in activities of alkaline phosphatases. 14 75

The protracted effect of ultrasound treatment on the parenchymatous organs of rats has been studied histochemically at 1 to 10-day intervals. As a result of the altered redox conditions it is the oxidative enzymes in the first place that react to ultrasound treatment and their normalization takes a long time. Exposure decreased the activity of lysosomal enzymes and in the cell membrane the alkaline phosphatase and ATP-ase too. The activity of hydrolases returned to normal parallel with the normalization of the microscopic structure.
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PMID:Effect of ultrasonic treatment on the organs of experimental animals. III. Enzyme-histochemical examination of the prolonged effect. 15 Feb 3

The physiopathological effects were studied of a common high erucic acid rapeseed oil as well as of Janpol, a low erucic acid oil produced of a rapeseed variety selected in Poland. Its erucic acid content equals 2.8% of total fatty acids. The studies were carried out on white male Wistar rats, 25 days old at the beginning of experiment. These animals were divided into 6 groups fed the diets in which 10 or 20% of kcal well supplied either by high erucic acid rapeseed oil, by Janpol rapeseed oil, or by the sunflower oil. The experiment lasted 6 months. Following parameters were determined: increase in body weight, the weight of selected organs, blood serum alkaline phosphatase and pseudocholinesterase activities, blood serum cholesterol and triglycerides level, the content of corticosterone in the adrenal glands and blood plasma. The liver was studied histochemically for the activity of acid and alkaline phosphatases and of ATP-ase, as well as for the presence of lipids. Morphological studies of the myocardium comprised macroscopic, histological and electron microscopic investigation. The low erucic acid rapeseed oil Janpol seems to evoke less disturbances than the high erucic acid one. Supplied in the amount corresponding to 10% of total calories intake the former exerts the effect on the biochemical and morphological parameters similar to that of sunflower oil. It can be thus assumed that the low erucic acid rapeseed oil Janpol can be used in the feeding of man when served in the amount lower than 10% of total calories.
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PMID:[Researches on the physiopathologic effects of rapeseed oil with high and low erucic acid content]. 15 22


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