Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.1.3.1 (alkaline phosphatase)
47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Whole cells of Mycobacterium avium, characterized by their negative response in the nine biochemical tests used for mycobacterial identification in our laboratory, turned positive for nitrate reductase, Tween-80 hydrolysis, beta-glucosidase, acid phosphatase, alkaline phosphatase, penicillinase, and trehalase after their wall portion was removed to yield spheroplasts. This suggested that the negative results in most of the biochemical procedures were caused by the exclusion mechanism at the wall level. Preliminary transmission and scanning electron microscopic studies showed differences at wall level between laboratory-maintained opaque, dome-shaped (SmD) and host-recycled smooth, transparent (SmT) colony type variants of M. avium and suggested the presence of an outer regularly structured layer in SmT variants. Comparative ultrastructural studies utilizing different polysaccharide coloration methods confirmed the presence of an outer polysaccharide layer in SmT variants which was probably related to their enhanced pathogenicity for experimental animals and drug resistance as compared to that of SmD variants. These findings are discussed with respect to multiple drug resistance, virulence, and gene expression of M. avium.
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PMID:Multiple drug resistance in Mycobacterium avium: is the wall architecture responsible for exclusion of antimicrobial agents? 679 25

Proximal and distal parts of the small intestine of 8-day-old suckling mice can best be maintained for 48 h in a serum-free organ culture system, Leibovitz L-15, at room air and room temperature. As determined by light and electron microscopy, the villous architecture was preserved as well as the classical ultrastructure of the enterocytes. Incorporation of 3H-thymidine and 3H-leucine continued during the culture period, reflecting a sustained synthesis of DNA and proteins for at least 48 h. The hydrolytic activities of the brushborder membrane, namely of lactase (L), trehalase (T), glucoamylase (GA) and alkaline phosphatase (AlPase) were measured in the explants as well as the culture medium. The overall enzymatic activities were increased as compared to the controls. In the tissue, L, GA and T activities remained stable or even increased during culture while in the medium an accumulation of enzymatic activities was noted especially for GA an AlPase. These results show that the morphological as well as the functional integrity of the mucosa is preserved for at least 48 h when small intestine of suckling mice is cultured in a serum-free medium.
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PMID:Organ culture of the small intestine of the suckling mouse in a serum-free medium. 683 27

Intestinal metaplasia of the human stomach was classified into two types, complete and incomplete. The complete type was associated with the intestinal marker enzymes sucrose alpha-D-glucohydrolase, alpha, alpha-trehalase, aminopeptidase (microsomal) (APM), and alkaline phosphatase (ALP). Tissue of this type contained goblet cells and Paneth's cells but not high-iron diamine (HID)-positive mucin staining with HID-Alcian blue. The incomplete type of intestinal metaplasia was associated with sucrose alpha-D-glucohydrolase, APM, goblet cells, and HID-positive mucin but not with alpha, alpha-trehalase, ALP, or Paneth's cells. For the examination of the distribution of the complete and incomplete types in 84, 27, and 16 resected specimens of human stomach with gastric carcinoma, gastric ulcer, and duodenal ulcer, respectively, disaccharidases were located with Tes-Tape. Specimens with intestinal metaplasia were divided into three classes: complete type only (class I), incomplete type only (class II), and a mixture of areas of the complete and incomplete types (class III). Of the 84 specimens from patients with gastric carcinoma, intestinal metaplasia was found in 76 (01%), and the percentages of specimens of classes I, II, and III were 32, 22, and 46, respectively. In these specimens, the percent incidence of class I increased and that of class II decreased with age. Of the 27 specimens from patients with gastric ulcer, 16 (59%) shopwed intestinal metaplasia and 10 of the 16 (63%) specimens were of class II. Of the 16 specimens from patients with duodenal ulcer, only 3 (19%) specimens showed intestinal metaplasia and all of them were of class II. The relationships of the complete and incomplete types of intestinal metaplasia to gastric carcinoma wre studied in 26 foci of minute carcinoma of the stomach less than 5 mm in largest diameter. Nineteen of 20 (05%) foci of the intestinal type of minute carcinoma were surrounded by intestinal metaplasia and 16 foci (80%) were surrounded by the incomplete type of intestinal metaplasia.
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PMID:Distribution of marker enzymes and mucin in intestinal metaplasia in human stomach and relation to complete and incomplete types of intestinal metaplasia to minute gastric carcinomas. 693 Dec 45

The development of intestinal brush border hydrolytic activities has been studied during thyroxine-induced metamorphosis of Rana catesbeiana. Alkaline phosphatase activity peaks at 3 and 10 days after the beginning of the thyroxine treatment. The cytochemical observations concerning alkaline phosphatase activity are in agreement with the biochemical data. At the ultrastructural level, alkaline phosphatase activity is particularly evident on the microvilli membranes of the enterocytes in the primary epithelium after 3 days and in the secondary epithelium after 10 days. gamma-Glutamyltranspeptidase exhibits an increase of activity between 7 and 10 days. On the other hand, glucoamylase, maltase, trehalase and leucylnapthylamidase activities decrease during thyroxine treatment, these enzymatic activities being lower than that normally observed after natural metamorphosis. The present study indicates that even though thyroxine is able to induce the morphological differentiation of the intestinal epithelium this hormone is unable to complete the enzymatic load of the new mucosa.
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PMID:Amphibian intestinal brush border enzymes during thyroxine-induced metamorphosis. 697 Jan 91

Purifications of mouse intestinal brush-border membranes from control explants and scrapings of intestinal mucosa have been compared. Based on the specific activity of sucrase used as a specific marker of these membranes, higher purification factors were obtained with control explants (24.7 +/- 0.9) as compared with scrapings of intestinal mucosa (14.8 +/- 0.9). However, similar patterns of proteins and enzymes were obtained by sodium dodecyl sulfate (SDS) - polyacrylamide gel electrophoresis after membrane solubilization by 2% SDS at room temperature. After 24 h of culture, higher molecular weight species of maltase-glucoamylase-isomaltase (band 4), alkaline phosphatase (bands 9-10), and trehalase (band 17) have been observed. Enzyme species appearing in the particulate fraction of culture media were, however, identical with those found at the brush-border membrane level in control explants, except for trehalase. These results are interpreted by considering the possible adsorption of serum components to brush-border membrane proteins. It thus appears that the membrane proteins and enzymes released in the media during organ culture are identical with those synthesized in the tissue in vitro or in vivo.
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PMID:Proteins and enzymes of the brush-border membrane of mouse intestine: influence of organ culture on gel electrophoretic patterns. 710 21

Urinary high molecular mass proteins (fraction P) solubilized in Triton X-100 and by papain have been compared with the solubilized human renal brush border membrane proteins. Crossed immunoelectrophoresis of Triton X-100 fraction P extract, by means of two polyspecific antisera directed against either renal membrane or fraction P, revealed eleven immunoprecipitates antigenically identical with detergent renal membrane antigens. Among them, five hydrolases were identified by zymogram staining: microvillus aminopeptidase, maltase, trehalase, gamma-glutamyltransferase and alkaline phosphatase. Eight papain-solubilized fraction P proteins and Triton X-100-solubilized membrane extract presented 'identity' patterns in tandem crossed immunoelectrophoresis, but differed in their amphiphilicity, as demonstrated by the change of precipitation pattern on charge-shift caused immunoelectrophoresis. Among the eleven detergent-solubilized fraction P antigens, nine were proved to be amphiphilic proteins and six presented bidirectional charge shifting properties similar to those of renal membrane antigens. Quantitatively, five detergent fraction P proteins were found in the same amounts as in renal membrane extract, two in lesser amounts and four in greater. Moreover, the same two plasma proteins were identified in fraction P as in the renal membrane. Thus important similarities exist between the urinary fraction P and the native renal membrane.
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PMID:Immunochemical analysis of high molecular mass urinary proteins. 712 88

Activities of intestinal enzymes were measured in genetically diabetic mouse of strain C57BL/KsJ-dbm to determine the long-term effects of genetic and uncontrolled diabetes on intestinal digestive function. Specific activities of enzymes were measured in intestinal homogenates, brush-border membrane fractions, and everted sacs from diabetic mice and their littermate controls. Sucrase, maltase, trehalase, alkaline phosphatase, and leucylnaphthylamidase activities were elevated in diabetes; lactase did not show any changes. The increases in disaccharidase activities in diabetes were in homogenates from both proximal and distal intestine but the increases in distal were more pronounced than in the proximal. Measurement of enzyme activities in brush-border membrane fractions showed a pattern similar to that observed in homogenates. Hydrolysis of sucrose and trehalose by everted sacs was markedly higher in the diabetic mice. It is therefore concluded that in genetic diabetes the digestive function of the intestine is stimulated, that the increased enzymes were incorporated into the brush-border membrane, and that the additional enzymes are accessible to the substrates in the intestinal lumen and so of physiological significance.
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PMID:Effect of genetic diabetes on enzymes of mouse intestinal brush-border membrane. 736 98

Suspensions of sequentially isolated villus and crypt cells were obtained in order to study certain biochemical changes associated with differentiation of epithelial cells in the small intestine of the mouse. Microscopic observation of the various cell fractions reveals that the epithelial cells detach as individual cells or small sheets of epithelium from the tip to the base of the villus, whereas cells in the crypt regions are separated as entire crypt units. The isolated cells retain their ultrastructural integrity as judged by electron miscroscopy. Chemical characterization of the various fractions shows that the total cellular protein content, expressed in activity per cell, remains relatively constant throughout the villus region followed by a noticeable drop in the crypt zone. On the other hand, sharp variations in values of cell DNA content are observed in the crypt zone depending on the reference of activity being used. Activity profiles of several brush border enzymes confirm the biochemical changes that occur during the migration of cells from the crypt to the villus tip, as observed in other species, with maximum activity of sucrase in the mid-villus region, of glucoamylase, trehalase, lactase and maltase in the upper third region, and of alkaline phosphatase at the villus tip. Forty-eight-hour suspension cultures of cell fractions corresponding to cells at the base of the villus and crypt zones show a moderate decrease in protein and enzyme activities to approximately 70% of their original value, with DNA content remaining stable throughout the incubation period. The use of biochemical activities as indicators of cellular integrity during cell culture is discussed.
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PMID:Characterization of isolated villus and crypt cells from the small intestine of the adult mouse. 741 92

Membrane proteins can be attached to the plasma membrane in several ways. Recently, a mechanism has been described, by which a number of cell surface proteins are anchored to the exoplasmic side of the plasma membrane by covalent linkage to glycosyl-phosphatidylinositol (GPI). The growth properties of renal epithelial cells in tissue culture enable free access to apical cell surface and brush border membrane proteins. To study the nature of membrane anchoring of apical plasma membrane enzymes in cultured renal epithelial cells, confluent LLC-PK1, OK, NRK, and MDCK epithelia were treated in tissue culture dishes with bacterial phosphatidylinositol-specific phospholipase C (PI-PLC), and the PI-PLC-specific release into the tissue culture medium of the apical membrane enzymes alkaline phosphatase (AP), gamma-glutamyl transpeptidase, leucine aminopeptidase, trehalase, and maltase was determined. Of the five enzymes tested, AP and trehalase, already described as GPI-anchored membrane proteins, were specifically released by PI-PLC from intact cell monolayers. Of the four cell lines investigated, LLC-PK1 cells express AP and trehalase which were released by PI-PLC. In OK cells, which lack AP activity, only trehalase was found to have PI-PLC-releaseable enzyme activity. MDCK cells, on the other hand, express AP activity, releaseable by PI-PLC, but no trehalase activity. In studies on the time course of synthesis and reinsertion of AP into the apical membrane of LLC-PK1 cells after removal by PI-PLC, a 60% recovery of AP activity was obtained only after 7 days. Analysis of protein release by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of culture supernatants after surface labeling with biotin and subsequent Western blotting with streptavidin revealed four protein bands at approximately 130, 90, 30, and 20 kD in LLC-PK1 cells and five GPI-anchored proteins at 110, 85, 65, 40, and 26 kD in OK cultures. The finding of a PI-PLC-specific release of apical membrane enzymes from renal tubular cell lines of different species (pig, opossum, rat, and dog) and of different nephron origin indicates a high conservation of the GPI anchor of renal brush border membrane proteins and further proves the high degree of differentiation retained by the cell lines in tissue culture. In addition, this method may provide a possible tool for isolating GPI-anchored apical membrane proteins from intact epithelial monolayer cultures.
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PMID:Selective release of apical membrane enzymes from cultured renal epithelia by phosphatidylinositol-specific phospholipase C. 750 39

The effects of epidermal growth factor (EGF) on neonatal intestines were examined in the rat. In 5-day-old rats, sucrase, trehalase, alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (gamma-GTP) activities in the small intestines were significantly increased after subcutaneous injection of EGF for 3 days (1 microgram/rat/day). gamma-GTP activity was also accelerated after oral EGF administration (2 micrograms/rat/day). Small intestines of 12-day-old rats injected with EGF for 10 days (1 microgram/rat/day) were significantly heavier than those of controls. These results suggest that EGF influences neonatal growth improving enlargement and functional development of their intestines.
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PMID:Effects of epidermal growth factor on neonatal growth of rat intestines. 791 Jul 14


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