Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The entire distribution of lymphatics in whole mount preparations of the Japanese monkey was studied using the enzyme-histochemical technique reported by KATO et al. (1990, 1991). In this staining, the lymphatic endothelium was colored dark brown by its positive 5'-nucleotidase activity, while most blood vessels (especially arterioles) were colored blue due to their positive alkaline phosphatase reaction. The whole mount preparations of the pleura treated enzyme-histochemically clearly indicated the distribution, branching patterns and running courses of lymphatic vessels. They revealed numerous short blind-ending knobs which represented the initial portions of lymphatics. These knobs were seen near the surface of the parietal pleura along its entire extent. In the costal and diaphragmatic pleura, the lymphatics ran parallel to the intercostal muscle fibers, but perpendicular to the tendinous and muscular fibers of the diaphragm; they formed ladders, independent of the courses of blood vessels. In the mediastinal pleura, lymphatic vessels showed a tree-like branching accompanying blood vessels. Under the light microscope, toluidine-blue stained semithin sections revealed the initial part of lymphatics as a small irregularly outlined cavity (7-10 microns in diameter) surrounded by a dense connective tissue. This lymphatic dilation was sometimes located close to a thin mesothelial layer. Such a structure suggesting a "stoma" was seen near the attachment of the muscular diaphragm to the sternum and along the borders of the ribs. Transmission electron microscopy revealed an occasional interruption in the mesothelium. This stoma continued to a submesothelial cavity whose base comprised an attenuated endothelium of an extended lymphatic vessel.
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PMID:Structure and distribution of the lymphatic vessels in the parietal pleura of the monkey as studied by enzyme-histochemistry and by light and electron microscopy. 129 50

The fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine, especially in the lamina propria of the ileocecal valve, was examined by light and electron microscopy using enzyme-histochemical staining. The distinction between the lymphatics and the blood vessels was made by light microscopy on cold glycol methacrylate resin (JB-4) sections using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining. The lymphatics were found to show strong 5'-Nase activity and to comprise irregularly shaped vessels or spaces. The central lymphatic vessels (central lacteals) in low villi were seen to lie deep within the ALPase-positive subepithelial capillary network. In the ileum side of the ileocecal junction, the 5'-Nase-positive lymphatics were seen both in the superficial layer and the deep layer of the lamina propria. On the contrary, in the cecum side the mucosal lymphatics were less numerous in the superficial layer and were distributed mainly in the deep layer near the lamina muscularis mucosae. These lymphatics ran through the lamina muscularis and merged into the lymphatic network in the submucosa. The submucosal lymphatics communicated with each other at the ileocecal junction and formed a well-developed network. Collecting lymphatics with valves were also seen near the tunica muscularis (sphincter muscle) in the deep submucosa. These lymphatics traversed the muscle layer and drained into the subserosal lymphatics.
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PMID:Enzyme-histochemical study on the fine distribution of the intramural lymphatics at the ileocecal junction of the monkey intestine. 180 44

Intralobular lymphatic vessels in the mouse thymus were demonstrated enzyme-histochemically by combined light and electron microscopy. In sections reacting to both 5'-nucleotidase (5'-Nase) and alkaline phosphatase (ALPase), the intralobular lymphatic vessels were identified as irregularly shaped spaces with strong 5'-Nase activity. The lymphatic vessels were closely associated with the branches of ALPase-positive intralobular arteries and veins. The initial lymphatics, which presumably originate from the perivascular spaces, were 5'-Nase positive. The distribution and intensity of the 5'-Nase activity in the lymphatic vessels revealed by light microscopy correlated well with those by backscattered image electron microscopy. The backscattered image scanning electron microscopy of the same area as observed under a light microscope more clearly highlighted the peculiar contours of lymphatic endothelial cells. Transmission electron microscopy showed that specific reaction product of the 5'-Nase after incubation in a medium containing L-tetramisole was predominantly localized on the outer surface of the lymphatic endothelial cell membranes.
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PMID:Enzyme-histochemical demonstration of intralobular lymphatic vessels in the mouse thymus. 225 33

The walls of lymphatics are characterized by strong 5'-nucleotidase activity, whereas those of blood capillaries reveal significantly lower or no activity. Alkaline phosphatase activity, on the other hand, is markedly higher in blood capillaries than in lymphatic vessels. On the basis of such characteristics, lymphatics and blood capillaries were distinguished histochemically in rat stomach using 5'-nucleotidase-alkaline phosphatase double staining. The distribution and intensity of lead-demonstrated 5'-nucleotidase activity in lymphatic vessels could be determined by comparing the images of the same histochemically stained cryostat section as seen by light and backscattered image scanning electron microscopy. The specificity of the 5'-nucleotidase reaction was obtained by inhibiting nonspecific alkaline phosphatase by including L-tetramisole in the 5'-nucleotidase incubation medium. The products of the 5'-nucleotidase activity were deposited on the outer surface of the plasma membrane of the lymphatic endothelial cells.
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PMID:Enzyme-histochemical identification of lymphatic vessels by light and backscattered image scanning electron microscopy. 237 10

Cytochemical differentiation between blood and lymphatic endothelium has been studied only in microvessels; 5'-nucleotidase (5'Nase) has been reported to be specific for lymphatic and alkaline phosphatase (ALPase) for blood endothelium. Adenylate and guanylate cyclase (AC and GC) have recently been proposed as lymphatic endothelial markers, but conflicting data exist. This study was designed to verify the presence of these enzymes in the endothelium of large vessels and to determine whether they are retained in endothelial cells (ECs) in culture. Segments of bovine mesenteric arteries, veins, and lymphatic collectors, and EC cultures obtained by collagenase treatment of the same vessels, were assayed for 5'Nase, ALPase, AC, and GC, and were observed by transmission electron microscopy. We found ALPase activity in blood and lymphatic vessels, and this was the only enzyme activity consistently retained under culture conditions. 5'Nase was found in lymphatic but not in blood endothelium, as previously reported for microvessels. AC and GC activity was found in blood but not in lymphatic endothelium. Hence, ALPase is not a useful marker to differentiate blood from lymphatic endothelium in large vessels, whereas 5'Nase is specific for lymphatic and AC and GC for blood endothelium. It is not clear why these enzyme activities are not expressed in culture.
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PMID:Cytochemical differentiation between blood and lymphatic endothelium: bovine blood and lymphatic large vessels and endothelial cells in culture. 791 6

Enzyme-histochemical methods of staining for 5'-nucleotidase (5'-Nase) and alkaline phosphatase (ALPase) were successfully applied to study the distribution and architecture of lymphatic vessels and their relationships to blood vessels in the rat stomach. Extensively lymphatic capillary networks were found in the gastric wall, but there were significant differences in their extent, pattern, distribution and structure in the four different zones: esophagus-stomach (E-S), forestomach-corpus (F-C), corpus-antrum (C-A) and antrum-duodenum (A-D). 5'-Nase-ALPase double staining revealed that the 5'-Nase-positive lymphatic vessels run in close proximity to ALPase-positive arteries and veins. The fine blood capillary network was located superficially to the lymphatic network within the same layer in the gastric wall. The abundant lymphatic network located in the deep lamina propria and the lamina muscularis mucosa was always closely associated with the base of the lowest gastric glands, and yet no interglandular lymphatic capillaries were encountered in the corpus or antrum. In contrast, fewer lymphatic capillaries were present in the lamina propria beneath the squamous epithelium of the forestomach. The distribution of the well-developed lymphatic networks with valve-like structures in the submucosa and subserosa exhibited typical features, i.e., the distribution was annular in the submucosa and fan-shaped in the subserosa in the antrum near the duodenum. Open junctions of lymphatic endothelial cells were seen in the deep lamina propria and submucosa. Collecting lymphatics containing valves were mainly located deep in the submucosa and subserosa. The deep lamina propria and submucosa may play a key role in lymph formation and interstitial tissue fluid homeostasis as well as in pathological processes in certain diseases. The present findings obtained by interstitially injecting ultra-fine carbon particle suspensions or Evans blue showed that a great deal of lymph drained into the lymphatics accompanying the left gastric artery. The existence of a forestomach may explain the complicated organization and constitution of lymphatic networks in the rat stomach.
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PMID:The distribution and architecture of lymphatic vessels in the rat stomach as revealed by an enzyme-histochemical method. 874 85

The existence of lymphatic vessels in the human dental pulp and their distribution were established by light and electron microscopy using an enzyme-histochemical method. The distinction between lymphatic and blood vessels was made by light microscopy on cryostat sections of undecalcified and decalcified teeth using 5'-nucleotidase(5'-Nase)-alkaline phosphatase double staining. On the tissue surface, 5'-Nase-positive lymphatic vessels were highlighted with good contrast and resolution by backscattered electron imaging using scanning electron microscopy. By transmission electron microscopy, dense granular precipitations resulting from the 5'-Nase reaction were seen on the luminal surface of the lymphatic endothelial cells as well as in the area at the basal side, but were absent in the blood vessels. These lymphatic vessels were more numerous in the central part than in the peripheral odontoblastic layer.
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PMID:Fine structure and distribution of lymphatic vessels in the human dental pulp: a study using an enzyme-histochemical method. 904 74

Intralobular lymphatics in the guinea pig pancreas were demonstrated enzyme-histochemically showing the extent, distribution and fine structure by combined light and transmission electron microscopy. 5'-nucleotidase(5'-Nase)-positive lymphatic vessels were present throughout the pancreas. Intralobular lymphatics among the acini were comparatively rare and generally independent of the blood capillaries, pancreatic ducts and acini. These lymphatics revealed the usual structural features, such as typical intercellular junctions and very tenuous vascular walls without continuous basal laminae. Fine precipitates of the cerium-based reaction product for 5'-Nase activity were found to be associated with cell membranes of the lymphatic endothelium and pinocytotic vesicles. Lymphatics were not closely related to the endocrine islets, although alkaline phosphatase(ALPase)-positive blood capillaries were well developed. Collecting lymphatic vessels with valves with weaker 5'-Nase activity were also detected in the interlobular connective tissue. ALPase activity, absent in the lymphatics, was positive in the blood capillaries, suggesting that it is also a useful way demonstrating, histochemically, the blood capillaries in the guinea pig pancreas.
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PMID:Demonstration of the intralobular lymphatics in the guinea pig pancreas by an enzyme-histochemical method. 927 54

The structural organization of the intramural lymphatic networks in the jejunum of the Japanese monkey (Macaca fuscata) was studied by an enzyme-histochemical method using whole-mount preparations of tissue layers. A distinction between the lymphatics and the blood vessels was made by light microscopy using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining. In each tissue specimen, the reaction product for 5'-Nase activity was localized in the lymphatic vessels, whereas that for ALPase activity was restricted to blood vessels. The mucosal layer exhibited an irregular network of tubular lymphatics connecting to the central lymphatics in the villi. The submucosal layer revealed a horizontal mesh-like network consisting of tubulo-saccular lymphatics. A dense maze-like network of lymphatics was distributed throughout the myenteric layer. The lymphatic networks were independent of the blood vessels. The present results indicate the usefulness of the enzyme-histochemistry of whole-mount preparations of the laminar layers for demonstration of the structure and distribution of the lymphatic system in the intestinal wall.
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PMID:Enzyme-histochemical demonstration of the intramural lymphatic network in the monkey jejunum. 937 69

The purpose of this study was to elucidate the morphometric changes occurring in hepatic lymphatics in human chronic viral liver diseases and to investigate the relationship between liver fibrosis, liver inflammation, and these changes. The lymphatic vessels were stained intensely by enzyme histochemistry for 5'-nucleotidase, whereas blood vessels stained well for alkaline phosphatase. We performed a morphometric analysis to estimate the number of lymphatic and blood vessels and their areas, using computer graphics software (NIH Image). Both the number of lymphatics in the specimens and their areas were increased according to the degree of liver fibrosis, but neither showed any relationship with the degree of activity of hepatitis. Neither the number nor the areas of the blood vessels showed any obvious relationship with the degree of fibrosis or the activity of chronic hepatitis. Correlation between clinical and laboratory data and the sizes and number of the lymphatics supported these morphological data. Our results clarified that the sizes and number of lymphatics are related to the stage of fibrosis in chronic viral liver diseases. This is thought to be due to increased lymph production, which is caused by the disturbance of the microcirculation associated with liver fibrosis.
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PMID:Morphometric analysis of lymphatic and blood vessels in human chronic viral liver diseases. 977 44


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