Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Disuse atrophy has been the subject of research studies of an animal model in which single-limb immobilization induces atrophic changes in the immobilized limb. These reveal systemic changes in the experimental animals that go far beyond the local response expected in that situation and are not fully understood as yet. We therefore performed a biochemical study on the effect of hind-limb immobilization on the serum and tissues of rats. The experiment was carried out on 70 young Sprague-Dawley male rats. In one group of 35 rats, the left hind-limb was immobilized for 3 weeks. Another group of 35 rats served as controls. Serum total protein, albumin, urea, creatinine, and calcium were found to be reduced during immobilization. Serum cholesterol levels, on the other hand, increased to a considerable extent. No changes were recorded with phosphate, bilirubin, and magnesium. Serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were both reduced in activity. The activity of muscle aspartate aminotransferase (AST) and bone alkaline phosphatase (ALP) was also decreased. Lactate dehydrogenase (LDH) remained unchanged in both serum and muscle. We discuss our findings in the light of previous knowledge regarding the atrophic process.
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PMID:Biochemical alterations secondary to disuse atrophy in the rat's serum and limb tissues. 1061 30

Disuse atrophy has been the subject of research studies that make the use of an animal model in which single-limb immobilization induces atrophic changes in the immobilized limb. The effect upon the nonimmobilized contralateral limb is, however, still unclear, and findings in the literature are inconclusive. We therefore performed a multidisciplinary study to clear this issue. The experimental population was 70 young male Sprague-Dawley rats. In one group of 35 rats, the left hind limb was immobilized for 3 weeks. Another group of 35 rats served as controls. limb and tibial bone weights (both "dry" and "ash") were found to be reduced in both hind limbs. Bone alkaline phosphatase (ALP) activity was likewise reduced in both limbs. We conclude that the contralateral hind limb in the rat is clearly subjected to atrophic changes that are similar in magnitude or severity to those experienced by the immobilized limb. We further discuss these findings in view of previous knowledge regarding the atrophic process.
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PMID:The effect of single hind-limb immobilization on the contralateral limb in the rat: a morphometric and biochemical study. 1061 62