Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

There are several reports on the weak contractile activity of uterus in the prolonged pregnancy. Some investigations showed that the weak contractile activity is associated with the low contents of RNA, proteins and glycogen in the myometrial cell. The material was obtained from caesarean sections in 27 patients with prolonged pregnancy and 30 patients with normal pregnancy. It was found a diminished contents of glycogen and lipids in the myometrial cell, and a decreased activity of phosphorylase and alkaline phosphatase but an increased activity of acid phosphatase. The lower content of energetic material and changes in the activity of the enzymes shows that the metabolism of the myometrial cell is impaired in prolonged pregnancy. One can suppose that the lowered level of estrogens is responsible for the described changes. The lower level of estrogens probably causes the decrease of 3'5' AMP in the myometrial cell with the impaired cell metabolism as a consequence. This may be one of the causes of a weak uterine contractile activity in the prolonged pregnancy.
 ...
PMID:[Histochemical studies of the myometrium in the prolonged pregnancy]. 121 Aug 81

The effect of dl-norgestrel (13 beta-ethyl-17 alpha-ethynyl-19-nortestosterone, Wy 3707) on the biochemical makeup of rabbit ovary and uterus was investigated. 20 adult, healthy virgin female rabbits either received olive oil only or olive oil plus 12 mcg of norgestrel/rabbit/day for 30 days. The animals were sacrificed 24 hours after the last treatment. In the ovary protein concentration and activities of glucose-6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and malate dehydrogenase (MDH) remained unaffected. However, in the uterus the level of protein was significantly elevated (p less than .01), the activities of G6PD and acid phosphatase were enhanced (p less than .05), and those of adenosine triphosphatase (ATPase) and alkaline phosphatase were diminished (p less than .05). LDH and MDH in the uterus remained unchanged. The effect of norgestrel at this antiovulatory dose in rabbits consisted of a disturbance in the biochemical constituents of the uterus leading to a lowering of the ATPase activity and an impairment of the anaerobic mechanism of the organ.
 ...
PMID:Effect of di-norgestrel (13beta-ethyl-17alpha-ethynyl-19-nortestosterone, Wy 3707) on the reproductive organs of rabbit. 122 53

The role of endometrial alkaline phosphatase in the mechanism of action of the IUD was investigated in the rat. A silk thread suture was inserted into the lumen of 1 horn of the rat uterus. Implantation sites were observed on Day 10 of pregnancy. Endometrial acid and alkaline phosphatase levels were measured in some of the animals on Day 5 of pregnancy. The suture significantly raised the endometrial alkaline (p .001) and acid (p .005) phosphatase levels in the horn with the IUD. When the IUD was removed on Day 2 of pregnancy a significant fall (p .001) in alkaline phosphatase was observed on Day 5 of pregnancy. 8 of 12 rats from whom the IUD had been removed on Day 2 showed evidence of implantation. It is suggested that the suture may be exerting its contraceptive effect by preventing implantation by raising endometrial alkaline phosphatase levels.
 ...
PMID:The role of endometrial alkaline phosphatase in the mechanism of action of the intrauterine device in the rat. 127 45

The refractory period that follows the period of sensitivity to a decidual stimulus in ovariectomized hormone treated mice was investigated. Medroxyprogesterone acetate provided constant progestin concentrations and silastic implants containing oestradiol maintained constant nidatory oestrogen concentrations. The nidatory stimulus was provided by crushing the uterus with a haemostat or by the intrauterine instillation of arachis oil. The decidual response was assessed by measuring changes in uterine weight or by examining the stroma for the presence of alkaline phosphatase. Sensitivity to the oil was first observed approximately 14 h after the insertion of the oestradiol implant but this sensitivity had waned by 32 h and was absent at 40 h. Crushing the uterus initiated a decidual response in mice treated with progestin alone but if the oestradiol implant was inserted then the uterus was responsive to crushing 24 h after insertion but not at 45 h. The traumatic decidual cell reaction (crushing), although not requiring nidatory oestradiol for its successful initiation, was nevertheless subject to the refractoriness that followed oestradiol sensitivity.
 ...
PMID:Timing of the window of uterine sensitivity to decidual stimuli in mice. 129 31

Using immunohistochemical method, the antigens were shown to be mainly located in the cecum, tegument, esophagus, pharynx, excretory bladder, uterus and oral sucker of Pagumogonimus skrjabini. Histochemical analyses showed that antigens contained enzymes such as acid phosphatase (ACP), alkaline phosphatase (AKP), and acetylcholinesterase (AChE). Other chemical substances, for example, alkaline protein, basic protein, glycogen and acid mucopolysaccharides were also detected in the antigens of P. skrjabini.
 ...
PMID:[Immunohistochemical localization and histochemical observation of antigens in Pagumogonimus skrjabini]. 130 40

A lysine-rich 18 kDa protein was isolated from bovine bone and examined for its effects on osteoblast-like MC3T3-E1 cells. This protein is homologous to a heparin-binding protein in brain and uterus. This protein enhanced cell attachment independent of the Arg-Gly-Asp cell-binding sequence and stimulated proliferation during the growth phase. Addition of this protein to cell cultures on days 11, 12, and 13 after confluency resulted in a 1.6-2.0-fold increase in the alkaline phosphatase activity and little increase in the DNA content. These findings suggest that the 18 kDa protein may be functional in promoting the proliferation and differentiation of osteoblasts.
 ...
PMID:Effects of a bone lysine-rich 18 kDa protein on osteoblast-like MC3T3-E1 cells. 151 Jun 62

We have developed a new detection method of deiminated proteins on polyvinylidene difluoride membranes. Citrulline residues in enzymatically deiminated histones were modified by incubating with diacetyl monoxime and antipyrine in a strong acid mixture. The products were injected to rabbits, and the antibodies obtained were affinity-purified using a modified citrulline column. Sample proteins blotted to the membrane were modified in a similar manner and incubated successively with the purified antibody and an alkaline phosphatase-conjugated second antibody. Detection was performed using a chemiluminescent substrate. The method enabled detection of 3-10 fmol of citrulline residues dot blotted as deiminated model proteins. It visualized numerous rat pituitary soluble proteins that had been enzymatically deiminated and Western blotted to the membrane. The data suggest usefulness of the method for detecting deiminated proteins regardless of the backbone protein molecules. Search for deiminated proteins on the Western blots of various rat tissue homogenates detected a single band on that of spinal cord, another band on that of uterus, and multiple bands on those of skin and hair root. The bands in the former two tissue homogenates comigrated with glial fibrillary acidic protein and vimentin, respectively.
 ...
PMID:Detection of citrulline residues in deiminated proteins on polyvinylidene difluoride membrane. 152 20

The relationship between the level of urokinase-type plasminogen activator (uPA) and pelvic lymph node metastasis was investigated in 20 patients with invasive cervical cancer of the uterus. Frozen sections from all surgical specimens were stained immunohistochemically by alkaline phosphatase anti-alkaline phosphatase method to detect uPA in cancer tissue. The concentration of uPA, determined immunologically, and the fibrinolytic activity were also examined in supernatants of homogenates of some cancer tissues. uPA was detected immunohistochemically in cancer cells of all specimens. A significant correlation was found between the extent of immunohistochemical staining for uPA and the concentration of uPA determined immunologically in cancer tissues (P less than 0.05). A positive correlation was also found between semiquantitative values determined by immunohistochemical staining for uPA and lymph node metastasis (P less than 0.05). Fibrinolytic activity in cancer tissues was confirmed by casein-plaque assay. These findings indicate that the pro-uPA/uPA contents in cervical cancer tissues are clinically useful for predicting the metastatic potential of these cancers to the pelvic lymph nodes.
 ...
PMID:Clinical significance of urokinase-type plasminogen activator (uPA) in invasive cervical cancer of the uterus. 152 11

An oral administration of antifertility drug Lyndral (17 alpha-ethinyl estradiol) at doses 5 micrograms/rat/a week for 3 consecutive weeks altered the internal biochemical milieu of uterus, showing a cyclic variation in both acid and alkaline phosphatase of the uterine fluid with high levels being present at proestrus and estrus in control rats. In Lyndral treated rats acid phosphatase showed a tendency to increase in estrus and metestrus rats, whereas alkaline phosphatase increased significantly during proestrus and estrus stages of the cycle. All these altered phosphatase levels in uterine fluid, after Lyndral treatment, gives some insight into the hormonal sensitiveness of these enzymes.
 ...
PMID:Cyclic variation in rats's uterine fluid phosphatase level after administration of lyndral (17 alpha-ethinyl estradiol). 166 77

At the University Women's Clinic in Kiel, the YAG contact laser has been used as a cutting instrument in pelviscopic operations since 1987. When the laser cuts, it produces only a scant amount of mechanical trauma. The determining factor is the amount of thermal damage produced along the wound margins and in direct neighboring tissue. The extent of the tissue change seen in the uterus and liver parenchyma of rats and the striated muscle of rabbits after application of the YAG contact laser was demonstrated using various staining techniques and stains. Liver parenchyma proved to be the most sensitive to thermal damage. In the uterine horn, enzyme-histochemical ATPase and alkaline phosphatase demonstrations showed a significantly wider zone of thermal damage after laser incision than did hematoxylin-eosin and Goldner staining techniques. A good understanding of the extent of thermal damage is essential for atraumatic pelviscopic operations using the YAG contact laser and also for the preventing of complications.
 ...
PMID:Width of thermal damage after using the YAG contact laser for cutting biological tissue: animal experimental investigation. 170 52


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>