Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This is a study of heat-stable alkaline phosphatase (HSAP65degreesC) concentrations in the serum of pregnant women with hypertension (42 cases), mild preeclampsia (40 cases) and severe pre-eclampsia (22 cases). The results are seen in relationship to the occurrence of intrauterine fetal death, growth retardation, intrauterine and neonatal asphyxia as well as the respiratory distress syndrome (RDS) in the newborn. The importance of a precise clinical classification of the patients is stressed. Pathological HSAP values are those which lie either over or under the normal range for HSAP activity. In addition "zig-zag" curves with values within the normal range are characterized as abnormal. Thus, serial estimations give the most reliable results. Serial estimations of HSAP are especially valuable in severe pre-eclampsia. Abnormal HSAP values in the 28th-38th week of pregnancy are a serious prognostic sign. False abnormal HSAP results were found in all 3 patient groups. One possible false normal HSAP curve also occurred.
 ...
PMID:Serum alkaline phosphatase in pregnancy. II. Serial HSAP65degreesC estimations in pregnancy complicated with hypertension and pre-eclampsia. 93 83

832 estimations of heat stable alkaline phosphatase (HSAP) and of heat alkaline phosphatase (HLAP) were carried out simultaneously in late pregnant women at 25th to the 42nd weeks of pregnancy. 147 of them delivered children with normal birth-weight. All these women suffered from pre-eclampsia, hypertension or any kind of superimposed pre-eclampsia. 110 other pregnant women with or without symptoms of pre-eclampsia gave birth to small for dates babies. In addition, the values of these patients were compared with 372 estimations of the same enzymes carried out in 120 patients with normal pregnancy and outcome of normal weighted children. The site of the values of every group showed no typical correlation to the course and outcome of their pregnancy. Regarding four special criterions it was possible to give a good prediction by serial determinations for the weight of the newborn in 80 per cent of the cases. A correlation between the urinary excretion of total oestrogens as well as HLAP and the values of HSAP was to be found only in some groups of patients.
 ...
PMID:[Behavior of serum alkaline during pregnancy. II. Pathological pregnancy]. 96 Nov 83

LDH isoenzymes and heat-stable alkaline phosphatase were studied in the serum and placental extract of 20 cases of pre-eclampsia and 10 normal pregnancies as a control. The starch-gel electrophoretic serum and placental isoenzymogram showed that LDH4 and LDH5 were the main isoenzymes in the placenta while LDH1 and LDH2 were the main isoenzymes in the serum in pre-eclampsia. The electrophoretic serum protein pattern in pre-eclamlobulins with decreased albumin fraction, while in the placenta, the albumin fraction was increased together with a decrease in the alpha-globulins. The electrophoretic pattern of serum alkaline phosphatase showed a main band of activity at the B-globulin zone in all cases of normal pregnancy and pre-eclampsia. In the placenta, two additional bands were detected.
 ...
PMID:Serum and Placental lactic dehydrogenase and alkaline phosphatase isoenzymes in normal pregnancy and in pre-eclampsia. 120 99

A comparison of the results of two assays for monitoring fetal well-being in normal and pathologic pregnancies is described. Concomitant leukocyte alkaline phosphatase (LAP) score determinations and 24-hour urinary estriol excretion were preformed in the same groups of pregnant women from the 31st week of pregnancy onward. There were 45 women with normal pregnancies, 10 women with antepartum death, 8 women in whom the membranes ruptured at least 30 hours before the determination, 40 women with moderate or severe preeclampsia and 26 women with postmaturity. Determination of LAP score is an easy, rapid and inexpensive method which can be performed in every laboratory. The results are as reliable as estriol determination. Thus LAP score may serve as a valuable method for evaluation of fetal well-being.
 ...
PMID:Leukocyte alkaline phosphate determinations and urinary estriol excretion in normal and pathologic pregnancies. 125 42

The subcellular localisation of acid and alkaline phosphatase has been studied in the trophoblast of placentae from both normal and complicated pregnancies. In placentae from uncomplicated pregnancies the number of trophoblastic acid-phosphatase-containing organelles decreases progressively as gestation proceeds whilst alkaline-phosphatase activity, although abundant at term, could not be demonstrated during the early stages of pregnancy. The acid-phosphatase-containing organelles are of two types; one is a small round body which is probably a lysosome whilst the other is a multivesicular body. The alkaline phosphatase is distributed mainly on the syncytial microvilli and plasma-membrane. It is suggested that the marked lysosomal activity during early pregnancy is related to the architectural refashioning of the placenta during this period and that there are two phosphatase-linked transfer systems in the trophoblast, one dependent upon acid-phosphatase-containing multivesicular bodies and being utilised during early pregnancy and the other reliant upon alkaline phosphatase and dominating during the second half of gestation. In placentae from prolonged pregnancies there is a further decrease in trophoblastic acid phosphatase and, usually, a continuing increase in alkaline-phosphatase activity. In placentae from babies of low birth weight this trend is sometimes reversed and alkaline-phosphatase activity either disappears or its reaction product diffuses throughout the syncytium; this is usually accompanied by a marked increase in the number of acid-phosphatase-containing multivesicular bodies. Placentae from women with pre-eclampsia show no loss of alkaline-phosphatase activity but are characterised by an increased number of lysosomal bodies.
 ...
PMID:An ultrahistochemical study of the distribution of acid and alkaline phosphatases in placentae from normal and complicated pregnancies. 126 21

The authors studied 74 pregnant women with preeclampsia presenting various degrees of gravity. The activity of alkaline phosphatase was determined cytochemically in the leucocytes. It was established that the activity of the enzyme was unchanged in mild preeclampsia and diminished progressively with advancement of the pathological process, compared with the respective values of normally progressing pregnancy at the same gestational period. A cytochemical criterium is proposed for differentiating the mild form of preeclampsia form the moderate form of preeclampsia.
 ...
PMID:[Alkaline phosphatase in the leukocytes of pregnant women with pre-eclampsia]. 237 92

Estimation of the alkaline phosphates isoenzymes in paired maternal serum and amniotic fluids in term uncomplicated pregnancies and in patients with pre-eclampsia, showed poor correlation coefficients between the levels of both heat stable and heat labile isoenzymes. There was a statistically significant fall in AF (P less than .05) HSAP in pre-eclampsia and a highly significant rise of HLAP in meconial liquor. It is concluded that the poor correlation between the levels of HSAP in maternal serum and amniotic fluid (despite their common source of origin), the normal levels of HLAP in maternal serum in the presence of significantly high levels of HSAP in maternal serum in the presence of significantly diminished levels in amniotic fluid point to a state of relatively diminished permeability of the chorioamniotic membranes to the alkaline phosphatase isoenzymes in Nigerians.
 ...
PMID:Observations on the alkaline phosphatase isoenzyme distribution in maternal and amniotic fluid compartments in Nigerian parturients. 335 45

To measure changes in bone alkaline phosphatase (EC 3.1.3.1) activity in serum as a function of duration of pregnancy, we adapted our existing alkaline phosphatase (ALP) isoenzyme assay (which has been used to measure bone, hepatic, and intestinal ALP activities in serum, in the absence of placental ALP) to allow quantification of individual ALP isoenzyme activities in the presence of placental ALP. The resulting CV for repeat measurements of bone ALP activity in artificial isoenzyme mixtures ranged from 23% for samples in which the bone isoenzyme represented 7% of total ALP activity to 11% for samples in which bone ALP accounted for 48% of total ALP activity. Values for repeat determinations of bone ALP activity in human serum samples (i.e., including samples obtained from pregnant women and from nonpregnant controls) varied by an average of 18%. We find, in initial applications of this method, that (a) the amount of bone ALP activity in serum is increased during pregnancy (P less than .001), and remains increased at six weeks postpartum, in non-lactating women (P less than .001), and (b) bone ALP activity at term was not significantly different in pregnant women with pre-eclampsia, diabetes, premature rupture of membranes, or premature labor, compared with normal pregnancies at term. Our data support the hypothesis that maternal bone formation may be increased during pregnancy.
 ...
PMID:Time-dependent changes in bone, placental, intestinal, and hepatic alkaline phosphatase activities in serum during human pregnancy. 366 32

Urinary excretion of the low molecular weight protein beta 2-microglobulin and tubular enzymes--alanine aminopeptidase (AAP), gamma-glutamyl transpeptidase (gamma-GT) and alkaline phosphatase (AP)--are very sensitive parameters for proximal tubular lesions. In patients with preeclampsia the renal excretion of beta 2-microglobulin allows to differentiate between a primary preeclampsia and a preeclampsia superimposed upon chronic pyelonephritis. In the first group the increase is 3- to 4-fold and in the second group up to 300-fold. In patients with kidney transplantation the urinary excretion of beta 2-microglobulin, AAP, gamma-GT and AP are several times higher than in normals. In case of a rejection episode a further increase of these proteins occur in more than 80% several days before clinical symptoms are present. The application of analgetics (paracetamol, acetylsalicylic acid) in healthy individuals in therapeutical dosages on 3 consecutive days does not show any tubular alteration by the measurement of urinary beta 2-microglobulin. Aminoglycosides (tobramycin, UK 18,892) lead to a cumulative increase of the renal excretion of beta 2-microglobulin and AAP while cephalosporins induce an increase of total proteins in the final urine under the same conditions.
 ...
PMID:Beta 2-microglobulin and other proteins as parameter for tubular function. 616 17

The aim of this work was to determine the levels of urinary human tissue non-specific alkaline phosphatase (hTNAP) in pre-eclampsia and eclampsia in order to assess renal tubular damage. Urine samples were collected from 26 mild pre-eclamptic, 26 were pre-eclamptic, 20 eclamptic patients and 20 healthy pregnant women (controls) in their late third trimester. Urinary hTNAP/creatinine (hTNAP/cr) in severe pre-eclampsia and eclampsia were significantly higher than in controls. Urinary hTNAP/cr was increased in 23%, 77% and 90% of cases of mild pre-eclampsia, severe pre-eclampsia and eclampsia, respectively, indicating that the increase correlates with the severity of the disease. Marked elevation or urinary hTNAP/cr was also associated with bad fetal outcome. These results provide additional evidence for renal tubular damage in pre-eclampsia and eclampsia.
 ...
PMID:Clinical significance of urinary human tissue non-specific alkaline phosphatase (hTNAP) in pre-eclampsia and eclampsia. 924 74


1 2 3 Next >>