Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Resistance to Marek's disease (MD) was selected over five generations in three strains and their four substrains. 2. MD mortality decreased consistently on natural exposure, but in birds artificially inoculated the mortality increased again in later generations. 3. Changes of gene frequencies at five polymorphic loci--blood group A, B and D, plasma esterase and alkaline phosphatase loci--were examined. 4. The frequencies of blood group A6 and B8 alleles showed a consistent trend of increase and the B11 allele gradually decreased in most strains. The Akp allele of alkaline phosphatase increased significantly in two of seven strains. 5. Comparisons of homozygotes and heterozygotes revealed a significant difference only at the A locus.
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PMID:Changes in polymorphic gene frequencies in strains of chickens selected for resistance to Marek's disease. 89 May 15

To improve sensitivity in the detection of Marek's disease virus (MDV) antigens in extracts of feather tips from infected chickens, we added a preformed streptavidin-biotin complex to the standard enzyme-linked immunosorbent assay (ELISA). Rabbit anti-chicken IgG-alkaline phosphatase that is used in the standard ELISA as the conjugate was replaced by a biotinylated rabbit anti-chicken IgG plus the streptavidin-biotin peroxidase complex (ABC) system. The ABC-ELISA system was correlated to the standard ELISA. There was increased sensitivity in the detection of MDV antigens present at low concentrations, while at the higher concentrations detection was similar to that in the standard ELISA. Both ELISA systems had the same increased sensitivity when compared with that of the agar gel precipitation (AGP) test.
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PMID:An improved ELISA method, using a streptavidin-biotin complex, for detecting Marek's disease virus antigens in feather-tips of infected chickens. 302 39

Studied was the polymorphism of alkaline phosphatase in blood plasma samples taken from 507 poulets and cocks of lines 66 and 77 of the Cornish breed, and lines 88 and 99 of the White Plymouth Rock breed. The birds were divided into three groups to test their resistance to Marek's disease: (1) controls, (2) infected with the virus of Marek's disease with 3 subgroups--resistant birds, slightly susceptible ones, and strongly susceptible ones, and (3) contacts, with two subgroups--survivals and birds that died. The blood plasma alkaline phosphatase was determined through horizontal electrophoresis on a starch gel, employing the method of Gahne. It was found that the alkaline phosphatase genetype FF frequency was lower with lines 66 and 77 than with line 88 and especially with line 99 of the White Plymouth Rock breed which proved most susceptible to the virus of Marek's disease. In the homozygotic alkaline phosphatase genetype SS none of the investigated lines showed any mortality. The resistance of broiler birds to Marek's disease was governed by the Akps allele, while the susceptibility was governed by the Akpf one. The former could be used as a genetic marker in the selection of initial lines of broiler birds by resistance, and the latter could be used as a marker in the selection by susceptibility.
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PMID:[Relationship between the genetic polymorphism of alkaline phosphatase and the resistance of broiler poultry to Marek's disease virus]. 629 72

Experiments were carried out with a total of 222 birds of the 5-A, 6-E, Ya-73, LL, and LS lines of the White Leghorn breed to find whether or not there existed any correlation between the alkaline phosphatase allels and the resistance to Marek's disease. The polymorphism of alkaline phosphatase was evaluated in the blood plasma of birds through horizontal electrophoresis in starch gel after G a hn e [3]. The resistance of the five egg laying lines of birds was found to be associated with the Akps allel, and their susceptibility - with the AkpF allel. No mortality with the homozygous gene type SS was established in the investigated lines except for a birds of the LL line. It was found that there existed full agreement of the results from the studies on broiler and laying lines concerning the exclusive resistance of birds of Akp SS gene type. This made it reasonable to believe that the indirect selection by resistance to the Marek's disease virus through the use of the Akps allel as a genetic marker was promising.
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PMID:[Genetic polymorphism of alkaline phosphatase and the resistance to Marek's disease virus]. 658 45

1. Two-way selection for delayed-type hypersensitivity wattle reaction (DWR) competence to BCG antigen was carried out over 4 generations, using a flock of White Leghorn chickens. DWR was measured by intradermal injection of BCG into the wattle of chickens. 2. Selection for DWR was effective. The generation means of average selection differential, selection response and realized heritability were 1.15 mm, 0.77 mm and 0.70 respectively. Correlated responses were found in body weight, egg weight and hatchability. In all of these traits, the L line (selected for low wattle reaction) showed higher values than the H line (high reaction). 3. Specific trends in gene frequencies were observed at the major histocompatibility B and alkaline phosphatase Akp loci. 4. Significant line differences were found in graft-versus-host reaction competence and Marek's disease resistance. In both characters, the L line was higher than the H line. No line differences were found in phytohaemagglutinin response, phagocytic activity or immune responses.
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PMID:Divergent selection for delayed-type wattle reaction of domestic fowls to BCG antigen. 819 90