Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.3.1 (alkaline phosphatase)
 47,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A two-site enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Gnathostoma spinigerum antigens in the sera of parasitized mice. This assay used IgG fractions prepared from serum of a G. spinigerum-infected rabbit as the capture antibody. The same IgG fractions were labeled with alkaline phosphatase and used as an antibody probe. The antigen detection assay was performed along with an antibody detection assay during the course of G. spinigerum infection in mice. Circulating antigen was detected after the first week of infection. The amount of detectable antigen increased steadily until the fourth week, but no significant amount of circulating antigen was detected thereafter. Serum antibody first appeared at the second week. Its level increased steadily until the fourth week, then remained high for at least eight weeks. The sensitivity of the two-site ELISA was approximately 6.75 ng/ml of larval somatic antigen and 27 ng/ml of excretory-secretory antigen. The assay gave false-positive results with Opisthorchis, Trichinella, and Angiostrongylus antigens at the level of 1, 728, 432, and 864 ng/ml or higher, respectively. This antigen detection assay may have application in the diagnosis of human gnathostomiasis.
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PMID:Detection of circulating parasite antigens in murine gnathostomiasis by a two-site enzyme-linked immunosorbent assay. 153 88

An ELISA for immunodiagnosis of human gnathostomiasis using a crude water extract of third-stage larvae of G. spinigerum as antigen, and alkaline phosphatase labelled goat antihuman IgG in the indicator system was developed and evaluated. At the titre of 1:400 and above positive results were observed in 100% of 4 parasitological confirmed and 10 eosinophilic meningo-encephalitis (EME) typical of gnathostomiasis cases, 56% of 160 cutaneous migratory swelling cases, 33% of 24 cases with EME typical of A. cantonensis infections, 23% of 92 cases with other parasitic infections and 1.5% of blood donors. The overall sensitivity was 59% and specificity 84%. The predictive value was 77%. The results indicated that ELISA is potentially useful for immunodiagnosis of gnathostomiasis but improvement of sensitivity and specificity is needed.
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PMID:ELISA for immunodiagnosis of human gnathostomiasis. 407 Dec

Ninety-eight out-patients of the Hospital for Tropical Diseases, Bangkok with clinical diagnosis of cutaneous gnathostomiasis were studied. All patients were treated with albendazole at a dosage of 400 mg (two tablets) twice daily for 14 days. They were seen periodically on day 0, day 14, day 28, day 195 and 1 year after treatment with laboratory investigations for any side effects of the treatment. There was a statistically significant increase of total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values when comparing the different periods. The abnormal results are clearly indicated in AST and ALT values (liver enzyme) especially on day 14 both male and female patients had highest levels. No significant association with time was found in ALP value.
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PMID:Changes of liver functions after albendazole treatment in human gnathostomiasis. 980 63

Comparative treatment of ivermectin in 21 patients (Group 1) and albendazole in 49 patients (Group 2) of gnathostomiasis gave the cure at 95.2% and 93.8% respectively. The ELISA OD and eosinophil counts were reduction after treatment. Side effects in ivermectin were hypotention, dizziness, weakness and diuresis; and side effects of albendazole were nausia, dizziness and increased alkaline phosphatase in two cases. Ivermectin should be an effective drug againts gnathostomiasis and more convenient in treatment single dose.
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PMID:Comparison of ivermectin and albendazole treatment for gnathostomiasis. 1112 42

A comparative study was performed for the treatment of gnathostomiasis patients with ivermectin 0.2 mg/kg for 2 days in 15 patients vs albendazole 400 mg twice daily for 21 days in 14 patients. The ivermectin and albendazole gave cure rates of 100% and 78.5%, respectively, however the difference was not statistically significant between the two drugs (Fisher's exact, p=0.0996). One year after treatment, the patients who had no migratory swellings and a drop in ELISA titers or a negative immunoblot test were considered to be cured. The side effect of ivermectin for two days was dizziness. The side effects of albendazole were nausea, dizziness, and an increased alkaline phosphatase.
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PMID:Double-dose ivermectin vs albendazole for the treatment of gnathostomiasis. 1612 32