Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.27.5 (RNase)
 17,967 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Haematoxylin-Basic fuchsin-Picric acid (HBFP) technique characterises two varieties of nuclear population in the rat hepatocytes. HBFP technique is capricious and the differentiation step should be controlled stringently; ethanolic picric acid, therefore, is recommended as a differentiation fluid. On the basis of controls treated with (1) ribonuclease (RNase), (2) deoxyribonuclease (DNase), (3) Bouins fluid or (4) protease, this study has determined that DNA-associated protein(s) and some DNA may be responsible for the HBFP produced nuclear fuchsinorrhagia. The heterogeneous nuclei of the rat hepatocytes were statistically analyzed in periportal, centrilobular and intermediate areas. Fuchsinorrhagic nuclei were preponderant in the periportal areas.
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PMID:On the mechanism of HBFP stain and an analysis of heterogeneous nuclei in rat hepatocytes. 9 26

The cerebellum of rat, rabbit, mouse, and bat were studied after staining with the Hematoxylin-Basic Fuchsin-Picric acid (HBFP) technique. In each species, heterogeneous Purkinje neurons became evident on the basis of red (i.e., fuchsinorrhagic) and blue (i.e., HBFP-negative) staining of cells. The former ranged from normal to atrophic-looking cells, while the blue staining Purkinje cells always showed typical perikaryal morphology with normal vesicular nucleus and prominent nucleolus. Staining differences were also seen by several different stains; however, the HBFP technique proved superior in the characterization of Purkinje cell heterogeneity owing to the tinctorial differences. The two classes of Purkinje neurons were still present after perfusion fixation. Electron microscopy demonstrated variable electron density in the Purkinje cells. Fuchsinorrhagic Purkinje neurons were absent in the 1-week old rats, but their number increased gradually thereafter. Tissue sections pretreated with RNase, DNase, or protease demonstrated that the fuchsinorrhagia of Purkinje neurons is a function of DNA/acidic protein complex. Since nucleic acids do not increase in Purkinje cells after maturation (i.e., after 8 weeks postnatal), the nucleic acids-associated-proteins, that are known to play a role in the regulation of gene expression, may be responsible for the fuchsinorrhagia of these Purkinje neurons.
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PMID:Histochemical and ultrastructural investigation of heterogeneous Purkinje neurons in mammalian cerebellum. 752 23