Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.27.5 (RNase)
 17,967 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Although IPN virus failed to multiply at 30 degrees, it replicated at 16 degrees and 22 degrees in SWT cells. At 22 degrees the viral eclipse period lasted nearly 6 hr with maximal virion titers attained by 24 hr, whereas replication at 16 degrees was much slower. The replication of the virion was inhibited by 0.05 mug/ml of AD which did not interfere with the production of reovirus. Biochemical studies revealed that cellular DNA synthesis was markedly reduced (greater than 50%) soon after infection whereas total RNA synthesis was enhanced. The period of rapid increase in RNA synthesis paralleled the exponential production of infectious virus. Viral inclusion bodies, revealed by acridine orange-staining of virus-infected cells (SWT and RGG-2) late in the infectious cycle, were found to contain single-stranded RNA on the basis of their staining characteristics and sensitivity to RNase.
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PMID:Replication of IPN virus: a cytochemical and biochemical study in SWT cells. 4 60

The RNA from infectious pancreatic necrosis virus has been purified and had a sedimentation velocity of 14S on sucrose gradients, a buoyant density of 1-60 g/ml in CS2SO4 and pyrimidine to purine ratios near unity. The RNA had the appearance of a linear double stranded molecule with an average length of 0-92 mum and a standard deviation of 0-07 mum when observed under the electron microscope using the Kleinschmidt protein film technique. This would correspond to a mol. wt. of 2-4 +/- 0-2 X 10(6). The RNase A resistance of IPN virus RNA exhibited a marked salt dependence; it was 92% resistant in 0-1 M-NaCl, but only 9% resistant, or less, in 0-*1 M-NaCl. The RNA was resistant to denaturation by boilding at NaCl concentrations of 0-04 M or higher, but did denature at lower concentrations. Polyacrylamide gel electrophoresis of the RNA indicated that two RNA species were present and the standard deviation of lengths in the electron microscope indicated that they could not differ by more than 4 X 10(5) in mol. wt.
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PMID:The structure of infectious pancreatic necrosis virus RNA. 55 73

In FHM and RTG-2 cells infected with IPN virus two types of viral directed RNAs where isolated in the presence of 0.5 mug/ml actinomycin D. One type, sedimenting at 14-15 S in sucrose gradients, was shown to be resistant to digestion by pancreatic RNase and did not precipitate in presence of lithium chloride 2 M. Furthermore the molecules sedimented in the same way as the double stranded RNA extracted from purified virions. The second type of RNA, sedimenting at 23-24 S, was sensitive to RNase and was precipitable by LiCl, indicating that it was single-stranded. Additionally it was shown by polyacrylamide gel electrophoresis that this type of RNA resolved into four peaks. The single stranded 23-24 S RNA was detectable in cells from the third hour following infection; it represents the major portion of total viral directed RNA that was synthesized during a cycle of replication. The synthesis of both single stranded and double stranded RNA increased exponentionally until the 7th hour post-infection and began to decrease at about 10 hours.
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PMID:[Study of viral specific RNA synthesis in FHM and RTG-2 cells infected with infectious pancreatic necrosis (IPN) virus (author's transl)]. 124 56