Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.27.5 (RNase)
 17,967 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To improve our understanding of the catalytic mechanism and regulatory properties of pyruvate carboxylase (EC 6.4.1.1), an important biotin-dependent enzyme, we have sought to isolate mutants in Saccharomyces cerevisiae which are defective in pyruvate carboxylase activity. One mutant was isolated which was unable to grow on glucose minimal medium unless supplemented with aspartate. Although the enzyme had only 25% of the wild type pyruvate carboxylase activity, Western analysis and RNase protection analysis demonstrated that the mutant gene was expressed at approximately 70% of the wild type level. On the basis of genetic crosses and complementation tests, we have attributed the defect to mutations in the PYC gene encoding pyruvate carboxylase.
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PMID:Isolation of a yeast mutant deficient in pyruvate carboxylase activity. 187 83

Bovine pyruvate carboxylase (PC; EC 6.4.1.1) cDNA was cloned by reverse transcription (RT) PCR. The coding region plus 3' untranslated region (UTR) of PC mRNA is 3926 bases and encodes 1178 amino acid PC precursor protein. A 5' rapid amplification of cDNA ends protocol was used to clone the 5' end of the mRNA. Six 5'UTR variants ranging from 68 to 363 bp were cloned. Bovine PC 5'UTR (bPC5') variants contain 68 (bPC5'A), 263 (bPC5'B), 363 (bPC5'C), 89 (bPC5'D), 275 (bPC5'E), and 178 bp (bPC5'F). All variants contain a common coding sequence. An RNase protection assay and RT-PCR analysis confirms the presence of the 5'UTR variants. The abundance of PC mRNA, determined by Northern blot analysis, indicates that PC is more abundant in gluconeogenic and lipogenic tissues where all PC variants are expressed compared with tissues that do not possess the full spectrum of PC transcripts. The data suggest that bPC5'A, bPC5'B, and bPC5'F are more abundant in bovine liver than the other variants.
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PMID:Cloning of bovine pyruvate carboxylase and 5' untranslated region variants. 1524