Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.27.5 (
RNase
)
17,967
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RNA tetraloops are common secondary structural motifs in many RNAs, especially ribosomal RNAs. There are few studies of small molecule recognition of RNA tetraloops although tetraloops are known to interact with RNA receptors and proteins, and to form nucleation sites for RNA folding. In this paper, we investigate the binding of neomycin, kanamycin, 2,4-diaminoquinazoline, quinacrine, and an aminoacridine derivative (
AD1
) to a GAAA tetraloop using fluorescence spectroscopy. We have found that
AD1
and quinacrine bind to the GAAA tetraloop with the highest affinity of the molecules examined. The equilibrium dissociation constant of the
AD1
-GAAA tetraloop complex was determined to be 1.6 microM.
RNase I
and lead acetate footprinting experiments suggested that
AD1
binds to the junction between the loop and stem of the GAAA tetraloop.
...
PMID:Binding of an aminoacridine derivative to a GAAA RNA tetraloop. 1550 Oct 63
