Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.27.5 (
RNase
)
17,967
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An immunoglobulin specific for
RNase
[EC 3.1.4.22] has been synthesized in a cell-free system containing either lymph node microsomes or polysomes or spleen polysomes from rats previously immunized with
RNase
. The synthesis of anti-
RNase
immunoglobulin was demonstrated by affinity chromatography using an
RNase
-Sepharose column. Supporting evidence for the cell-free synthesis of immunoglobulin was obtained by separating the synthesized immunoglobulin from other proteins by DEAE-Sephadex A-25 column chromatography and by neutralization of
RNase
activity with the separated immunoglobulin.
Lymph nodes
and spleen had an almost equal capacity to synthesize the immunoglobulin to
RNase
. Under our experimental conditions, 5 to 15 per cent of the total protein synthesis was directed toward immunoglobulin synthesis.
...
PMID:Demonstration by affinity chromatography of the cell-free synthesis of ribonuclease-specific immunoglobulin. 112 23
