Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.27.4 (ribonuclease)
 6,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

These studies describe the normal anatomical distribution of neurons containing the mRNA coding for neurotensin (proneurotensin/neuromedin N) in the rat forebrain and midbrain and examine how that distribution is altered by acute administration of the dopamine antagonist haloperidol. A novel fluorescence detection method was developed and employed with biotinylated oligonucleotides to permit the rapid, sensitive visualization of in situ hybridization. The hybridization was temperature-sensitive, eliminated by ribonuclease, and co-localized in neurotensin-immunoreactive perikarya in the midbrain. In the forebrain of control rats, proneurotensin mRNA-containing neurons were found in the dorsomedial and ventrolateral caudate/putamen, in the nucleus accumbens, in the ventral striatum including the olfactory tubercles, and in the septal nuclei. Haloperidol induced significant increases in the frequencies and distributions of hybridization-positive neurons in the striatum and septal nuclei. In the midbrain, the highest frequency of hybridization-positive neurons occurred in the substantia nigra and the superior colliculus. Prominent populations were also present in the dorsal and ventral periaqueductal gray, the oculomotor region, and the medial longitudinal fasciculus. Less prominent were populations of neurons in the dorsomedial deep mesencephalic nuclei and the ventral tegmental area. Haloperidol induced only modest increases in the frequency of pro-neurotensin mRNA-containing neurons in the ventral tegmental area, and had no effects elsewhere in the midbrain. These results show that the fluorescent detection techniques used in this analysis provide a very rapid, reliable method for localizing hybridized mRNA in the rat brain. This study also suggests that a subpopulation of striatal neurons begin to express proneurotensin mRNA in response to haloperidol treatment. This effect of haloperidol on striatal neurons contrasts with results from additional studies of enkephalin mRNA in the striatum, suggesting that the mechanisms of haloperidol stimulation may differ between neurotensin and enkephalin-containing neurons.
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PMID:The effect of acute haloperidol treatment on brain proneurotensin mRNA: in situ hybridization analyses using a novel fluorescence detection procedure. 216 9

Expression of the gene encoding neurotensin (NT/N) is developmentally regulated in the adult small bowel with maximal expression noted in the distal ileum; the mechanisms responsible for this strict spatial-specific expression pattern are not known. The purpose of this study was to determine whether NT/N expression is altered by ileojejunal transposition. Rats underwent either sham operation or ileojejunal transposition and were killed 2 months after operation. The transposed (either jejunum or ileum) and sham-operated segments of gut were removed, a portion was processed for histologic examination, and the remainder was extracted for total RNA and analyzed by ribonuclease protection using a rat NT/N probe. For comparison, expression of another gut endocrine gene, peptide YY, and an enterocyte-specific gene, sucrase-isomaltase (SI), was also determined. Expression of the gut endocrine genes, NT/N and peptide YY, were minimally affected by transposition of either the jejunum or ileum. In contrast, SI expression was markedly altered in both the transposed jejunum and ileum compared with corresponding sham gut segments. Expression of the NT/N gene is minimally altered after jejunoileal transposition despite marked adaptive and morphologic changes in the transposed segments. These findings provide further support that the strict pattern of NT/N expression is "imprinted" in the gut and maintained regardless of location along the cephalocaudal gut axis.
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PMID:Effect of gut transposition on the expression of the endocrine gene neurotensin. 984 79