Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.27.4 (ribonuclease)
 6,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Lysyl oxidase, an extracellular enzyme involved in the maturation of collagen and elastin, also appears to function as a phenotypic suppressor of transformation by the ras gene product, p21. Genomic clones of the mouse lysyl oxidase gene have been isolated, analyzed, and sequenced. Lysyl oxidase appears to be a single-copy gene, organized into seven exons and six introns, and spans approximately 14 kb of the mouse genome. The gene encodes two messages, sized at about 4.8 and 3.8 kb, that differ in the length of the untranslated sequence at the 3' end of the gene. All of the 3' untranslated sequence and the polyadenylation signals are contained in exon VII; there is no evidence of alternate splicing. Primer extension and ribonuclease protection experiments revealed two sites of transcription initiation in a region with sequence motifs characteristic of a promoter, which was upstream and adjacent to the 5' untranslated sequence found in the cDNA.
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PMID:Structure of the mouse lysyl oxidase gene. 810 Feb 14

The Aspergillus cell wall contains most of the antigens secreted by the fungus during its active in vitro or in vivo growth. These antigens, which bind to the IgE and IgG of allergic and aspergilloma patients or are secreted in the biological fluids of patients with invasive aspergillosis, are of primary importance in the diagnosis of aspergillosis. Located at the interface between host and pathogen cells, the fungal cell wall plays a major role during fungal invasion. It contains several surface receptors involved in adhesion of the fungus to host proteins and cells. Some of the wall antigens are also directly involved in the colonization of the host tissues by the fungus. Very few of these putative virulence factors have been purified until now. A 33-kDa alkaline protease of the subtilisin family can hydrolyze several extracellular matrix proteins such as collagen, fibrinogen, elastin. However, gene disruption experiments have shown that protease-deficient mutants are still able to infect mice. An 18-kDa antigen, which has been detected in the urine of patients with invasive aspergillosis, is present in vivo in the lung of mice infected with A. fumigatus. It has a ribonuclease activity that cleaves a single phosphodiester bond in a highly conserved region of the ribosomal RNA. Its role in the virulence of A. fumigatus has not been demonstrated until now. Biochemical and molecular characterization of the wall antigenic aggressins should be pursued.
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PMID:Cell wall antigens in Aspergillus fumigatus. 829 77

The temporal changes in the expression of fibronectin and other extracellular matrix genes were studied in rat aortic rings incubated in vitro in a serum-free medium. Changes in all forms of fibronectin mRNA increased progressively during the 24-hour incubation period, although an increase in the alternatively spliced form of fibronectin designated EIIIA was most pronounced. Both collagen and elastin mRNA levels decreased markedly during the 24-hour interval, as did alpha-actin mRNA. The increase in the relative amount of the EIIIA isoform after a 24-hour incubation was also shown using ribonuclease protection assays. In situ hybridization showed the distribution of the induced fibronectin mRNA to be within all cell types, including endothelial cells, medial smooth muscle cells, and adventitial fibroblasts. Localization in the media was not uniform and was clearly identified mainly in clusters of cells distributed throughout the media. The early induction of fibronectin mRNA was inhibited by genistein, implicating tyrosine kinase activation as a causative factor in fibronectin expression. The in vitro changes reported may reflect a phenotypic change in vascular cell types that is both similar to and different from the changes reported in vivo under conditions in which vascular injury and repair occur.
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PMID:Selective induction of an embryonic fibronectin isoform in the rat aorta in vitro. 837 Jan 23