Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.27.4 (ribonuclease)
 6,621 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Myobacterium tuberculosis. J. Bacteriol. 91:2146-2154. 1966.-The ribosomal fraction of the attenuated strain, H37Ra, of Mycobacterium tuberculosis was treated with trypsin alone, ethylenediaminetetraacetic acid (EDTA) alone, EDTA and pancreatic ribonuclease, or with trypsin and ribonuclease. After each of these treatments, the ribosomal fractions were injected intraperitoneally into male CF-1 mice to test their capacity to produce an immune response to infection with virulent tubercle bacilli, strain H37Rv. Removal of protein with trypsin left the immunogenicity unchanged; EDTA alone reduced immunogenicity in the smaller vaccinating doses; EDTA plus ribonuclease reduced the immunogenicity by approximately 50% in the highest (1.0 mg) vaccinating dose; ribonuclease alone, after treatment with trypsin, reduced immunogenicity also approximately 50%. A crude mycobacterial ribonucleic acid (RNA) was prepared by extraction of the ribosomal fraction with alcohol. This RNA preparation was as effective in producing an immune response as the ribosomal fraction from which it was prepared, unless the RNA was partially or completely degraded during the preparation. The effect of ribonuclease on the immunogenicity of the RNA was similar to that obtained with the ribosomal fractions, except that ribonuclease completely destroyed the immunogenicity of a partially degraded RNA. RNA appears to be an essential part of an immunizing substance in attenuated tubercle bacilli, which produces a high degree of immunity in mice; 50 mug (dry weight) will protect approximately 80% of the mice, and as little as 0.5 mug will protect approximately 30% of the mice. Mycobacterial RNA not incorporated in Freund's incomplete adjuvant was nonimmunogenic. Yeast RNA incorporated in Freund's incomplete adjuvant was not immunogenic.
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PMID:Effect of trypsin and ribonuclease on the immunogenic activity of ribosomes and ribonucleic acid isolated from Mycobacterium tuberculosis. 495 10

Moore, Dorothy E. (University of Chicago, Chicago, Ill.), and James W. Moulder. Autoradiographic study of deoxyribonucleic acid synthesis in L cells infected with the agent of meningopneumonitis. J. Bacteriol. 92:1128-1132. 1966.-L cells infected with the agent of meningopneumonitis were labeled with H(3)-cytidine at 5-hr intervals after infection, and cell samples were fixed every 5 hr after labeling. These preparations were then digested with ribonuclease, stained by the Feulgen procedure, and examined by autoradiography. Labeled meningopneumonitis inclusions were first seen 15 hr after infection. Deoxyribonucleic acid (DNA) was synthesized in both L-cell nuclei and meningopneumonitis agent for as long as 40 hr after infection. Nuclear DNA synthesis was unaffected until 25 hr after infection, at which time synthesis of agent DNA reached its peak. After 25 hr, both meningopneumonitis and L cell DNA synthesis declined.
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PMID:Autoradiographic study of deoxyribonucleic acid synthesis in L cells infected with the agent of meningopneumonitis. 592 41

Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.), and Guy P. Youmans. Nature of the labile immunogenic substance in the particulate fraction isolated from Mycobacterium tuberculosis. J. Bacteriol. 88:1030-1037. 1964.-Deoxyribonuclease had no effect on the immunogenic activity of the labile particulate fraction isolated from ruptured viable cells of the H37Ra strain of Mycobacterium tuberculosis, but decreased the ropiness of the ruptured cellular mass. Ribonuclease, in a high concentration, decreased the immunogenic activity slightly. Addition of yeast ribonucleic acid to particulate fraction incubated at 37 C prevented the decrease in immunogenic activity which normally occurs at this temperature, suggesting that endogenous ribonuclease may be involved in the reduction of activity. Differential centrifugation by the use of Brodie's (1962) method showed that the particles which sedimented at 56,550 x g were immunogenically active. Experiments were done to determine whether the integrity of the structure of the particle was necessary for immunogenic activity. It was found that sonic oscillation, freezing and thawing several times, the addition of surface-active agents (sodium lauryl sulfate or deoxycholate), and preparation of the particulate fraction in hypotonic solutions either decreased or destroyed immunogenic activity. This strengthens the evidence that a structural unit is necessary for activity. In addition, both a waxy sediment and the smallest particles which sedimented only at 144,000 x g were highly immunogenic if incorporated into Freund's incomplete adjuvant. In the absence of adjuvant, neither produced any immunity.
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PMID:NATURE OF THE LABILE IMMUNOGENIC SUBSTANCE IN THE PARTICULATE FRACTION ISOLATED FROM MYCOBACTERIUM TUBERCULOSIS. 1421 15

Schlenk, F. (Argonne National Laboratory, Argonne, Ill.), and J. L. Dainko. Action of ribonuclease preparations on viable yeast cells and spheroplasts. J. Bacteriol. 89:428-436. 1965.-Recrystallized pancreatic ribonuclease releases more than half of the ultraviolet-absorbing cellular constituents of Candida utilis and Saccharomyces cerevisiae. The presence of salts and buffer interferes with the action of the enzyme on the cytoplasmic membrane, and intracellular constituents show a similar inhibitory effect. Polymerized ribonuclease and conformational isomers cannot penetrate the pores of the yeast cell wall, but they are effective on the membrane of spheroplasts. All forms of the enzyme interfere strongly with the viability of the cells.
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PMID:ACTION OF RIBONUCLEASE PREPARATIONS ON VIABLE YEAST CELLS AND SPHEROPLASTS. 1425 11