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Query: EC:3.1.27.3 (RNase T1)
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Infectious retroviruses have been isolated from gibbon apes and a woolly monkey. Previous studies have shown that these isolates share some antigenic determinants and that they exhibit partial nucleic acid homology. To further define the relationships in this group of viruses, we compared the RNAs of the viruses of the woolly monkey-gibbon ape class by two-dimensional polyacrylamide gel electrophoresis of the large RNase T1-resistant oligonucleotides. The degree of sequence identity between the RNAs was determined by the similarity of the fingerprint patterns and in some cases by partial sequence analysis of individual oligonucleotides. This technique permitted us to determine the degree of sequence identity in related RNA species. These studies showed that as much as 80% of the genomes of gibbon ape leukosis virus-Halls' Island and gibbon ape leukosis virus-brain could be identical. The other viruses, simian sarcoma-associated virus, gibbon ape leukosis virus-Thailand, and gibbon ape leukosis virus-San Francisco, showed an extensive but somewhat lower degree of sequence identity (between 40 to 60% of the genomes.
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PMID:Structural analysis of the genomes of gibbon ape and woolly monkey leukosis viruses. 22 47

Retroviruses have been isolated from the tissues of human leukemia patients. Previous studies have shown that these isolates share some antigenic determinants with the family of viruses isolated from the woolly monkey and gibbon ape and that they exhibit partial nuclei acid homology with this same group of viruses. We have compared the RNAs of the viruses by two-dimensional polyacrylamide gel electrophoresis of the large RNase T1-resistant oligonucleotides. The degree of sequence identity between the RNAs was determined by the similarity of their RNase T1-resistant oligonucleotide pattern on gels, fingerprints, and in some cases by partial sequence analysis of individual oligonucleotides. This technique permits us to determine the degree of sequence identity among related RNA species. From our studies we conclude that viruses isolated from the tissues of two human leukemia patients, A1476 and SKA 21-3, as well as some subcultures of a virus isolated from the leukemic tissues of a third patient, HL23V, are closely related to the wooly monkey virus. However, the fingerprints of other HL23 viral isolates are very similar to that of GaLVSF, a gibbon ape leukemia virus isolated from a lymphosarcoma.
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PMID:Relationship of retroviruses isolated from human leukemia tissues to the woolly monkey-gibbon ape leukemia viruses. 624 70

RNAs of representative viruses of the exogenous simian sarcoma virus-gibbon ape lymphosarcoma virus (SiSV/GALV) and endogenous baboon virus (BaEV) classes of subhuman primate type C viruses were compared and related to HEL-12 virus, an isolate derived from human embryonic lung cells. The extent of sequence identity between different viral RNA preparations was determined by comparison of fingerprint patterns obtained after electrophoretic separation of RNase T1-resistant oligonucleotides. The studies presented indicate that HEL-12 viral RNA and simian sarcoma-simian associated virus [SiSV(SSAV)] RNA share 90 to 95% of the large oligonucleotides. From 5 to 10% of virus-specific oligonucleotides were detected in each of several virus preparations examined and their occurrence was independent of the cell line on which the virus ws propagated. HEL-12 virus and GALV-SF have 50% unique oligonucleotides in common. These are the same oligonucleotides that are shared between GALV-SF and SisV(SSAV) RNA. Two BaEV isolates, M7 and BILN, and RD114, and BaEV-related endogenous virus of cats, each easily displayed distinguishable oligonucleotides patterns. Large oligonucleotides characteristic for these three endogenous virus isolates were not detected in the fingerprints of HEL-12 virus, SiSV(SSAV) and GALV-SF.
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PMID:Comparative oligonucleotide analysis of exogenous and endogenous primate type C viruses. 626 79