Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.27.3 (
RNase T1
)
1,228
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A comparative study of the association of two ribonucleases, barnase and
binase
, with the
polypeptide
inhibitor barstar has been performed by the Brownian dynamics simulation method. It was shown that the method adequately reproduced the dependence of the association rate on pH and ionic strength of solution and the influence of mutations of some ribonuclease amino acids. Two types of energetically favorable complexes of
binase
-barstar encounter were determined. In the type I complex, the amino acids of
binase
active center take part in the complex formation. In the second complex, the active center is free. It was supposed that the temporary binding of barstar into complex of type II is competitive relative to the inhibition reaction. This can partially explain the decrease in the rate of
binase
inhibition as compared with the corresponding reaction of barnase.
...
PMID:[Brownian dynamics simulation of the association of Bacillus amyloliquefaciens ribonuclease (barnase) and Bacillus intermedius ribonuclease (binase) with barstar]. 1663 28
A comparative study of the competitive reactions-the association reaction of
binase
with
polypeptide
inhibitor barstar and the reaction of
binase
dimerization-has been performed by the Brownian dynamics simulation method. It was shown that three types of the
binase
dimers could be formed and the dimerization reaction could compete with the inhibition reaction. The first type of the dimers leaves the active centre of
binase
free. During the formation of the dimers of the second and the third types the active centre of one or both
binase
molecules is blocked and ribonuclease becomes partially or fully inactive. Brownian dynamics simulation shown, that the ratio of competitive reaction rates depends on pH and ionic strength of solution.
...
PMID:Brownian dynamics simulation of the competitive reactions: binase dimerization and the association of binase and barstar. 1765 90
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