Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.27.3 (
RNase T1
)
1,228
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The low temperature structural transition (low leads to high) of 5 S RNA from Escherichia coli is investigated by partial digestion with
ribonuclease T1
. In addition to a general masking of guanines from the nuclease, differential changes of accessibility are observed when Mg2+ and salt concentrations are increased to bring about the low leads to high transition. Residue G13 becomes more exposed in the high form while residues G54, G56, G61,
G72
, and G83-86 become less exposed. The observed cutting rate at other sites is unchanged. A possible conformational change is discussed which could explain the observed changes in
RNase T1
digestion patterns as well as the physical chemical observations.
...
PMID:A characterization of the low temperature structural transition of Escherichia coli 5 S RNA by partial enzymatic digestion. 619 17
