Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.27.3 (RNase T1)
 1,228 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have isolated a replication-defective rapidly transforming sarcoma virus (designated 16L virus) from a fibro-sarcoma in a chicken infected with td107A, a transformation-defective deletion mutant of subgroup A Schmidt-Ruppin Rous sarcoma virus. 16L virus transforms fibroblasts and causes sarcomas in infected chickens within 2 wk. Its genomic RNA is 6.0 kilobases and contains sequences homologous to the transforming gene (fps) of Fujinami sarcoma virus (FSV). RNase T1 oligonucleotide analysis shows that the 5' and 3' terminal sequences of 16L virus are indistinguishable from (and presumably derived from) td107A RNA. The central part of 16L viral RNA consists of fps-related sequences. These oligonucleotides fall into four classes: (i) oligonucleotides common to the putative transforming regions of FSV and another fps-containing avian sarcoma virus, UR1; (ii) an oligonucleotide also present in FSV but not in UR1; (iii) an oligonucleotide also present in UR1 but not in FSV; and (iv) an oligonucleotide not present in either FSV, UR1, or td107A. Cells infected with 16L virus synthesize a protein of Mr 142,000 that is immunoprecipitated with anti-gag antiserum. This protein has protein kinase activity. These results suggest that 16L virus arose by recombination between td107A and the cellular fps gene.
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PMID:Isolation of 16L virus: a rapidly transforming sarcoma virus from an avian leukosis virus-induced sarcoma. 628 31

The avian Fujinami sarcoma virus (FSV) contains a hybrid transforming gene (delta gag-fps) with a 5' 1.3-kb portion derived from the gag gene of avian retroviruses and a 3' 2.8-kb portion (fps) derived from a cellular prototype. A lambda recombinant DNA clone carrying fps sequences within a 16-kb insert of cellular DNA, termed lambda proto-fps clone 12, has been selected from a chicken DNA library for comparison with the viral onc gene. Mapping of endonuclease-resistant proto-fps DNA fragments and hybridization with cloned viral DNA located FSV-related sequences at the 3' end of the insert within a region of about 4.25 kb. Alignment of endonuclease-resistant proto-fps and viral DNA fragments relative to common RNase T1-resistant oligonucleotide sequences of viral RNA, identified by fingerprinting DNA-RNA hybrids, indicated: (i) that proto-fps is colinear with viral fps but is interrupted by 1.75 kb of scattered sequences unrelated to viral fps; (ii) that among the nine endonuclease sites compared, proto-fps and viral fps share one PvuII, one BamHI, and possibly a Kpn1 site at homologous locations, and that they each have unique endonuclease sites and common sites at unique locations; (iii) that within 12 kb upstream from the 5' boundary of overlap with viral fps, proto-fps lacks gag-related sequences; and (iv) that proto-fps clone 12, like several others isolated by us, lacks at the 3' end an equivalent of the 3' 10 to 20% of viral fps. The eight endonuclease site-map coordinates of proto-fps and viral DNA also divided 44 fps oligonucleotides of viral RNA into 9 map segments. We conclude that the onc gene of FSV differs from proto-fps in delta gag and in multiple point mutations, compatible with a transforming function for the viral gene and a normal function for the cellular sequence homolog. Since proto-fps is unrelated to essential virion genes, the onc gene of FSV must have originated from cellular proto-fps by rare, illegitimate recombination.
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PMID:Structural relationship between the chicken DNA locus, proto-fps, and the transforming gene of Fujinami sarcoma virus, delta gag-fps. 631 Aug 87


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