Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.27.1 (
RNase
)
16,360
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Circular RNAs (circRNAs) are crucial regulators in human cancers, including nonsmall cell lung cancer (NSCLC). In this study, we aim to explore the biological functions and molecular mechanisms of circ_0074027 in NSCLC.
Methods:
Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to determine the expression of circ_0074027, paired like homeodomain 1 (
PITX1
) mRNA, microRNA-335-5p (miR-335-5p), and
cullin 4B
(
CUL4B
) mRNA. The feature of circ_0074027 was analyzed by
RNase
R digestion assay. Flow cytometry analysis was adopted to analyze cell cycle and cell apoptosis. Cell counting kit-8 (CCK-8) assay and colony formation assay were performed to assess cell proliferation. Western blot assay was conducted to measure protein levels. Dual-luciferase reporter and RNA pull-down assays were carried out to verify the relationships among circ_0074027, miR-335-5p, and
CUL4B
. The murine xenograft model was established to investigate the role of circ_0074027
in vivo
.
Results:
High expression of circ_0074027 was found in NSCLC tissues and cells. Circ_0074027 knockdown suppressed cell viability, cell cycle process, and colony formation and promoted apoptosis in NSCLC cells
in vitro
and inhibited tumor growth
in vivo
. Circ_0074027 acted as a sponge of miR-335-5p. The effect of circ_0074027 knockdown on NSCLC progression was weakened by miR-335-5p inhibition. Moreover,
CUL4B
was a target gene of miR-335-5p.
CUL4B
overexpression reversed the inhibitory effects on cell viability, cell cycle process, and colony formation and the promotional effect on cell apoptosis caused by miR-335-5p in NSCLC.
Conclusion:
Circ_0074027 facilitated NSCLC cell progression through regulating miR-335-5p/
CUL4B
axis.
...
PMID:Circ_0074027 Contributes to Nonsmall Cell Lung Cancer Progression by Upregulating
CUL4B
Expression Through miR-335-5p. 3258 May 76
