Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.27.1 (RNase)
 16,360 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Tomato plants grown in commercial greenhouses in Sonora, Mexico, developed either yellow mosaic, leaf curling, and stunting (phenotype 1; 20 to 35%) or chlorosis and a feathery appearance of leaves (phenotype 2; 15 to 25%) in December 1997 and again in October 1998. Polymerase chain reaction (PCR) with diagnostic primers (prAV324 and prAC889) that amplify an approximately 576-bp fragment of the coat protein (CP) gene of whitefly-transmitted geminiviruses indicated the presence of a begomovirus. Biolistic inoculation of tomato seedlings with RNase-treated extracts of three symptomatic tomato samples, each, resulted in reproduction of disease symptoms. With PCR primers prAV2644 and prAC1154 (1), the entire CP (approximately 776 bp) and its flanking sequences were amplified from extracts of symptomatic tomato, and amplicons were cloned and sequenced. Comparisons of a minimum of three CP gene sequences from each phenotype revealed the presence of at least two begomoviruses. The phenotype 1-associated CP gene shared 92.6, 86.2, and 85.3% identity with the CP sequence of chino del tomate (CdTV) [AF106936], tomato mottle, and abutilon mosaic geminiviruses, respectively. The CP sequence associated with phenotype 2 was 94.6, 77.1, and 77.1% identical to pepper hausteco (PHV) [X70418], bean golden mosaic-Guatemala (BGMV-GU), and Texas pepper (TPV) gem-iniviruses, respectively. Previously, CdTV was reported from tomato in Chiapas, Morelos, Sinaloa, and Tamaulipas, Mexico, while PHV has been identified in Guanajuato, Quintana Roo, Sinaloa, and Tamaulipas, Mexico, and in Texas (2). This is the first report of CdTV-like (>92% identity) and PHV-like (>94% identity) geminiviruses associated with greenhouse-grown tomatoes in Sonora, Mexico. References: (1) A. M. Idris and J. K. Brown. Phytopathology 88:648, 1998; (2) I. Torres-Pacheco et al. Phytopathology 86:1186, 1996.
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PMID:First Report of Chino del Tomate and Pepper Hausteco Geminivurses in Greenhouse-Grown Tomato in Sonora, Mexico. 3084 95

Tupanviruses are giant viruses recently discovered in Brazil from extreme environments: Tupanvirus soda lake (TPV-SL) and Tupanvirus deep ocean (TPV-DO). Unexpected features in Tupanviruses is the cytotoxic effect observed during infection, where the virus degrades the ribosomal RNA (rRNA) of its amoebal host. Interestingly, only TPV-SL causes this rRNA shutdown. We performed a genomic comparison of the two strains to determine potential modifications explaining the absence of rRNA degradation by TPV-DO. Whole genome comparisons were performed as well as more in-depth analysis at the gene level. We also calculated selective pressure on the orthologous genes between the two viruses. Our computational and evolutionary investigations revealed a potential target: a ribonuclease T2. These enzymes are known to be involved in cellular RNA catabolism such as in lysosomal degradation of rRNA. Our results suggest a functional ribonuclease localized in acid compartment closely related to ribonuclease T2 from eukaryotes. Silencing of the RNAse T2 gene of TPV-SL abolished its rRNA shutdown ability thereby correlating in silico assumption to the experimental evidence. In conclusion, all our results pointed to RNAse T2 as a target for explaining the difference for rRNA degradation ability between both strains.
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PMID:How Tupanvirus Degrades the Ribosomal RNA of Its Amoebal Host? The Ribonuclease T2 Track. 3284 55