Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.26.9 (ribonuclease)
 6,589 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of ultraviolet irradiation on Escherichia coli 30-S ribosomal subunits were studied. At the doses of radiation used in this work (0-4.5 x 10(5) quanta/30-S subunit), only protein S7 was found to be significantly crosslinked to the 16-S RNA. In conditions where 25% of the protein was covalently crosslinked, the ability of the irradiated 30-S subunits to reassociate with 50-S subunits and their activity in polyphenylalanine synthesis decreased strongly. Similar results were obtained by irradiation with a germicide lamp (254 nm) or with a monochromatic ultraviolet light at 248 nm. No additional proteins were crosslinked to the 16-S RNA by irradiating 30-S subunits depleted in protein S1 or 70-S ribosomes. The covalent complex of 16-S RNA and protein S7 was isolated and digested by T1 ribonuclease. The oligonucleotide remaining attached to the crosslinked protein was characterised as A-C-C-U-C-G [position 1261 - 1266, see the sequence published by Carbon et al. (1979) Eur. J. Biochem. 160, 399-410]. Analysis of this fragment suggests that protein S7 was linked to the cytosine at position 1265 in the RNA sequence.
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PMID:Effect of ultraviolet irradiation on 30-S ribosomal subunits. Identification of the RNA region crosslinked to protein S7. 698 1

Acid alizarin violet N in an acidified aluminum potassium sulfate solution (AAV) is presented as a nuclear fluorochrome. We demonstrate using 1 N HCl, deoxyribonuclease, and ribonuclease digestion methods that this stain has specificity for nucleic acids similar to other aluminum mordant stains in 95% ethanol-fixed material. The method presented gives stable preparations and is resistant to fading for at least two years. Strong fluorescence of AAV stained material is detected under conventional mercury vapor lamp and argon ion laser illumination. AAV stained confocal scanning laser microscope (CSLM) images are collected in the red channel of the microscope (detecting lambda > 600 nm), there being no AAV emission in the green channel (detecting lambda 527-565 nm). The xanthene dyes eosin Y and dichlorofluorescein are used as counterstains and can be imaged in both channels. We present a method for use with the CSLM, utilizing double imaging techniques.
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PMID:Aluminum acid alizarin violet: a general purpose nuclear fluorochrome. 945 75

We have synthesized two low molecular weight organic molecules, PY and IN successfully, which selectively stain nucleolus and cytoplasm of living cells in 30 min, with a much lower uptake in the nucleus. Nucleic acids electrophoresis and digest test of ribonuclease indicate their markedly higher affinity for RNA, especially PY. Moreover their RNA localization in cells is further supported by digest test of ribonuclease, namely, the nucleolar fluorescence signal is distinctly lost upon treatment with RNase. And, the fact that live cells stained by PY and IN still possess physiological function can be confirmed: 1) MTT assay demonstrates that the mitochondria of cells stained remains its electron mediating ability, 2) Double assay of PY/IN and propidium iodide as well as trypan blue testing show that the membrane of cells stained still is intact. Importantly, compared with the only commercial RNA probe, SYTO RNA-Select, PY and IN exhibit much better photostability when continuously illuminated with 488 nm laser and mercury lamp. These results prove that PY and IN are very attractive staining reagents for visualizing RNA in living cells.
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PMID:Low molecular weight fluorescent probes with good photostability for imaging RNA-rich nucleolus and RNA in cytoplasm in living cells. 2433 61