Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The localization and expression of
chondromodulin-I
(
ChM-I
), an angiogenesis inhibitor, in the rat articular cartilage during maturation from 2 to 10 weeks of age were examined by immunohistochemistry, Western blot analysis and
ribonuclease
protection assay, and the results were compared with those in the epiphyseal cartilage.
ChM-I
was found to be diffusely immunostained in the inter-territorial space of the cartilage matrix from the intermediate to the deep layers at the immature stage. As the articular cartilage matured, the immunoreactivity was localized around the hypertrophic chondrocytes in the deep layer and the immunoreactivity became weak after maturation. In contrast, the
ChM-I
immunoreactivity was intense in the epiphyseal cartilage at all ages examined.
ChM-I
was detected by Western blotting as a broad band or occasionally as a cluster of multiple bands (approximately 25 kDa) in both the articular and the epiphyseal cartilage. The intensity of the bands decreased gradually with age in the articular cartilage, but was unchanged in the epiphyseal cartilage at all ages. Ribonuclease protection assay revealed that
ChM-I
mRNA also decreased gradually with age in the articular cartilage in parallel with the maturation of the articular cartilage, while no decrease in
ChM-I
mRNA was found in the epiphyseal cartilage. The expression of
ChM-I
mRNA in the articular cartilage was less than that in the epiphyseal cartilage at all ages. The decrease in amount of
ChM-I
in the mature articular cartilage suggests that
ChM-I
plays a more important role in the maintenance of avascularity in the immature articular cartilage than in the mature one. The avascular condition may be preserved by angiogenic inhibitors or mechanisms other than
ChM-I
in the mature articular cartilage.
...
PMID:Chondromodulin-I expression in rat articular cartilage. 1452 63
The localization and expression in the rat cornea of
chondromodulin-I
(
ChM-I
), an inhibitory angiogenesis factor, were examined by immunohistochemistry, Western blot analysis,
ribonuclease
protection assay, and real-time PCR assay. We found immunoreactivity for
ChM-I
in the epithelial layer but not the stromal layer or endothelial layer in the cornea, in addition to the positive
ChM-I
immunoreactivity in other sites in the eye such as the sclera, retina, and ciliary body. The
ChM-I
immunoreactivity was most intense at the outside of the basal cells and in their cytoplasm while the intensity of the immunoreactivity decreased gradually from the wing cells to the superficial cells in the corneal epithelial layer. No reactivity however, was detected in the Bowman's membrane or conjunctival epithelial cells which had continuity with the corneal epithelial cells. The expression of
ChM-I
mRNA was demonstrated in the cornea at one-third less intensity than that in the sclera with choroids and retinal pigment epithelium by
ribonuclease
protection assay and real-time PCR.
ChM-I
in the corneal epithelial layer may prevent neovascularization and maintain avascularity in the cornea.
...
PMID:Localization and expression of chondromodulin-I in the rat cornea. 1501 47
