Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effects of estradiol treatment, which stimulates cell division in rat uterine epithelial cells, on the in vivo expression of
heparin-binding epidermal growth factor
(
HB-EGF
), cyclin D1, and cyclin B1 messenger RNA (mRNA) in these cells have been examined using
ribonuclease
protection assays. Estradiol gave rise to significant increases in steady state levels of
HB-EGF
2 and 24 h after treatment. Cyclin D1 mRNA levels were elevated 8 and 10 h after estradiol administration, corresponding to the G1 phase of the mitotic cycle, and cyclin B1 mRNA was only expressed 16-24 h after estradiol treatment, which corresponds to the G2 and M phases of the rat uterine epithelial cell cycle. Estradiol-stimulated increases in
HB-EGF
mRNA were not affected by treatment with cycloheximide, but were inhibited by the estrogen antagonist compound, ICI 164,384, demonstrating that the estrogen-stimulated increase in
HB-EGF
mRNA is a primary, estrogen receptor-mediated response of rat uterine epithelium to estradiol. Progesterone treatment, which blocks epithelial cells in G1 of the cycle, suppressed levels of
HB-EGF
mRNA below those observed in ovariectomized rats. These results indicate that
HB-EGF
mediates the regulatory effects of both estradiol and progesterone on rat uterine epithelial cell proliferation through an effect on the production of G1 phase molecules such as cyclin D1.
...
PMID:Mediators of estradiol-stimulated mitosis in the rat uterine luminal epithelium. 949 26
