Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have developed a rapid and sensitive method for detecting
ribonuclease
(RNAase). The method makes use of a RNA-
Pyronine Y
complex which has a different absorption spectrum from that of
Pyronine Y
alone. When the RNA is hydrolyzed by RNAase, the spectrum of the complex changes to that of unbound
Pyronine Y
. The resultant decrease in absorbance at 572 nm is linear for final RNAase concentrations ranging from 2 to 45 ng/ml. Optimal assay conditions were 11.5 micrograms/ml
Pyronine Y
, 0.56 mg/ml RNA, 80 mumol/ml Tris-HCl buffer, pH 7.8, and 2-45 ng/ml RNAase. The effect of complex concentration, pH, molarity and temperature upon the rate of the reaction were determined. The assay is applicable to crude cell-free extracts.
...
PMID:A simple spectrophotometric method for the measurement of ribonuclease activity in biological fluids. 666 3
