Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
1. The effect of chemical modification of
ribonuclease
on its reaction with
ribonuclease
inhibitor has been studied. 2. Removal of free amino groups from the enzyme with
nitrous acid
or by acetylation did not affect the reaction. Some changes altered the stoicheiometry of the reaction and
ribonuclease
S was found to be inhibited linearly by increasing amounts of
ribonuclease
inhibitor, in contrast with ribonuclease A, which is inhibited in a non-linear way. One derivative of
ribonuclease
containing dimethylaminonaphthalenesulphonyl groups actually reacted with
ribonuclease
inhibitor to a greater extent (and linearly) than did the unaltered enzyme. 3. The positively charged histidine at the active site and the active enzyme did not appear to be necessary for the reaction since 1-carboxymethylhistidine-119-
ribonuclease
reacted with
ribonuclease
inhibitor to almost the same extent as the native enzyme. In general, any significant change in the conformation of
ribonuclease
was accompanied by a loss in its ability to combine with inhibitor. The presence of 8m-urea also prevented reaction of
ribonuclease
with inhibitor. 4. Some characteristics of the reaction of
ribonuclease
inhibitor,
ribonuclease
and deaminated
ribonuclease
with RNA and deaminated RNA were investigated.
...
PMID:The reaction of ribonuclease inhibitor with modified ribonucleases. 597 72
