Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.26.9 (ribonuclease)
 6,589 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A suitable histochemical method able to discriminate vitamins D and 7-dehydrocholesterol from cholesterol and its esters is proposed taking into account the alkaline permanganate-toluidine blue reaction, the alkaline permanganate-Schiff reaction and the inhibitory effect of the HgCl2-formalin fixative. This method appears specific according to the results provided by spot test. On tissue sections the alkaline permanganate-toluidine blue reaction is more sensitive and more specific than the alkaline permanganate-Schiff reaction. A previous ribonuclease treatment or the methylation effect improves the specificity of the alkaline permanganate-toluidine blue reaction. The 2.4-dinitrophenylhydrazine blockade must be used previously to the alkaline permanganate-Schiff reaction as an attempt to improve its specificity. The previous hydrolysis carried on by some suitable treatments seems very useful to detect masked vitamins D and 7-dehydrocholesterol. The oxidized products accountable for the reactivity of vitamins D and 7-dehydrocholesterol to the alkaline permanganate-toluidine blue and alkaline permanganate-Schiff reactions were discussed.
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PMID:A histochemical method able to detect vitamins D and to discriminate it from cholesterol and its esters. 241 84

The peracetic acid-toluidine blue and the peracetic acid-Schiff reactions as well as the failure of HgCl2-formalin fixative for inhibiting these reactions appears as a very useful method for free cholesterol histochemical detection and to discriminate it from its esters and from some of its metabolic products such as 7-dehydrocholesterol and vitamins D. The cholesterol histochemical detection provided by those reactions appears specific taking into account the findings afforded by spot tests. The peracetic acid-toluidine blue reaction is very suitable for histochemical purposes on tissue sections, since it does not produces tissues damages, it is sensitive and the stained end product is almost insoluble in the solvents frequently used in histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-Schiff reaction. The peracetic acid-toluidine blue reaction is more sensitive and more specific than peracetic acid-Schiff reaction, since the tissues do contain Schiff reactive products unable to be 2,4-dinitrophenylhydrazine blocked which interfere on the results of the latter reaction decreasing its specificity and its sensitivity. The cholesterol histochemical detection based on the peracetic acid-toluidine blue and peracetic acid-Schiff reaction as it is suitable in discriminating free cholesterol from its esters as well as from some of its metabolic products it appears very useful for metabolic studies on tissue sections.
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PMID:A histochemical method suitable to discriminate free cholesterol from its esters and both from 7-dehydrocholesterol and vitamins D. 642 30

Hormone-sensitive lipase expression was studied in the human colon adenocarcinoma cell line, HT29. Diacylglycerol lipase and cholesterol esterase [corrected] activities in HT29 cells were inhibited by known inhibitors of hormone-sensitive lipase (diethyl-p-nitrophenyl phosphate, NaF and HgCl2) to the same extent as in human adipocytes. A polyclonal antiserum directed against rat hormone-sensitive lipase inhibited 89% of HT29 cell lipase activity. HT29 hormone-sensitive lipase was the same size as the adipocyte enzyme as was its mRNA. Complete homology between mRNA sequences in HT29 and adipocyte was demonstrated using ribonuclease protection assay. These data are consistent with the expression of a protein closely related, if not identical, to the enzyme expressed in human adipose tissue. HT29 is the first human cell line where hormone-sensitive lipase expression has been shown.
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PMID:Expression of hormone-sensitive lipase in the human colon adenocarcinoma cell line HT29. 769 73

A ribonuclease with a molecular weight of 29 kDa as determined by FPLC-gel filtration on Superose 12 was isolated from the sclerotia of the mushroom Pleurotus tuber-regium using a procedure involving extraction with aqueous buffer, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and FPLC on Mono S. The protein was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and CM-cellulose. It was homodimeric, made up of two identical subunits, each with a molecular weight of 14.5 kDa as witnessed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It exhibited potent ribonucleolytic activity toward Poly G. Its ribonucleolytic activity was resistant to heating at 100 degreesC for 30 min, but was inhibited by HgCl2, ZnSO4, NiSO4, CaCl2, and Pb(NO3)2.
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PMID:A ribonuclease from sclerotia of the edible mushroom Pleurotus tuber-regium. 978 79