Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Isolation of high-quality RNA from
ribonuclease
-rich tissue such as mouse pancreas presents a challenge. As a primary function of the pancreas is to aid in digestion, mouse pancreas may contain as much a 75 mg of
ribonuclease
. We report modifications of standard phenol/guanidine thiocyanate lysis reagent protocols to isolate RNA from mouse pancreas.
Guanidine thiocyanate
is a strong protein denaturant and will effectively disrupt the activity of
ribonuclease
under most conditions. However, critical modifications to standard protocols are necessary to successfully isolate RNA from
ribonuclease
-rich tissues. Key steps include a high lysis reagent to tissue ratio, removal of undigested tissue prior to phase separation and inclusion of a
ribonuclease
inhibitor to the RNA solution. Using these and other modifications, we routinely isolate RNA with RNA Integrity Number (RIN) greater than 7. The isolated RNA is of suitable quality for routine gene expression analysis. Adaptation of this protocol to isolate RNA from
ribonuclease
rich tissues besides the pancreas should be readily achievable.
...
PMID:RNA isolation from mouse pancreas: a ribonuclease-rich tissue. 2514 27
