Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The interactions between ribonuclease A and solvent components in aqueous
2-methyl-2,4-pentanediol
(MPD) have been investigated by differential refractometry and light scattering at pH 5.8, i.e., conditions similar to those used to crystallize the protein from this solvent system. Application of multicomponent thermodynamic theory shows that, at all solvent compositions up to 50% (v/v) MPD, the protein is preferentially hydrated; i.e., addition of
ribonuclease
to the mixed solvent leads to an increase in the chemical potential of MPD. This unfavorable thermodynamic interaction leads to phase separation, probably caused by local salting out of the MPD by the charges on the surface of the protein molecule. A parallel examination by circular dichroism (CD) has shown that the CD spectrum of
ribonuclease
in 50% MPD is indistinguishable from that in dilute buffer.
...
PMID:Interaction of ribonuclease A with aqueous 2-methyl-2,4-pentanediol at pH 5.8. 2 25
The crystals of a complex between
ribonuclease
Ms, the extracellular
ribonuclease
from Aspergillus saitoi, and 3'-guanylic acid were obtained from
2-methyl-2,4-pentanediol
solution by vapor diffusion technique in the hanging drop mode. The crystals belong to orthorhombic space group P2(1)2(1)2(1) with dimensions a = 47.0 A, b = 62.8 A, c = 37.9 A. The crystals diffract strongly up to at least 2.0 A resolution.
...
PMID:Crystallization of a complex between ribonuclease Ms and 3'-guanylic acid. 254 76
