Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.1.26.9 (ribonuclease)
 6,589 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Solutions of a number of proteins were subjected to gentle agitation in the presence of small quantities of decane. 2. Some of the protein was lost from solution and adsorbed on the surface of the emulsion formed; at the same time some of the decane was bound to the protein remaining in solution. 3. The two processes were found to be related and a mechanism is proposed to explain the relationship. 4. With lysozyme and ribonuclease, the protein in the aqueous phase of an emulsion exhibited normal enzymic activity, whereas the fraction adsorbed on the interface was much less active but recovered activity on desorption.
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PMID:Protein-hydrocarbon interactions. Interactions of various proteins with pure decane. 498 65

Several fungal strains ofAspergillus andPenicillium were immobilized by cryopolymerization in polyvinyl alcohol cryogel beads.Aspergillus clavatus was the best producer of extracellular ribonuclease. Enzyme productivity and growth of free and immobilized cells in shake flasks and agitated bioreactor were studied. Ribonuclease production and growth behaviour depended on concentrations of glucose, peptone and soybean in the culture medium. Enzyme production was influenced by agitation and aeration intensity. In repeated batch, shake-flask cultures, the immobilized cells showed 2 to 3.5 times higher enzyme activity than free cells. The optimal conditions in a bioreactor were at 150 rev/min agitation speed and 0.5 vol/vol.min aeration. Enzyme productivity of immobilized cells (237 units/g dry mycelium.h) was 2.1 times higher than the productivity of free cells in a bioreactor, and 2.3 times higher than that of a shake-flask culture.
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PMID:Ribonuclease production by free and immobilizedAspergillus clavatus cells. 2441 34