Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.1.26.9 (ribonuclease)
 6,589 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Purified FMD virus was heat treated and then examined by electron microscopy with the negative contrast technique. Fibrils of varying length and thickness were present; they were not present after ribonuclease treatment. They were similar to the fibrils described for other picornaviruses. Viral RNA was the essential component of these artificially arranged fibrils, which were composed of threads about 10 A wide, separated from one another by a gap of about 10 A.
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PMID:[Electron microscopy demonstration of fibrillar structures in the foot-and-mouth disease virus]. 17 50

The main immunogenic component of FMD virus harvests is the intact 140S virus particle. For the production of stable vaccines it is important to ensure that virus strains having a stable capsid should be used. The method of measuring the heat stability of FMD virus by following the increase in optical density of purified virus during capsid breakdown as the temperature is increased was described in 1964 (P. Bachrach, 1964, J. Mol. Biol 8. 348). We have investigated this technique in the hope that it might provide a means of screening virus isolates rapidly to eliminate unstable strains. Using this method we have been unable to follow the course of virus breakdown from the absorbance profile alone. A much more precise indication has been obtained by including ribonuclease in the virus sample. This enhances the optical density rise which occurs when the capsid disintegrates and the RNA becomes accessible. A sigmoid curve is obtained from which 50% breakdown temperature can be calculated. The presence of RNase does not appear to alter the temperature at which breakdown occurs since parallel samples heated in the absence of RNase showed the same behaviour when virus breakdown was measured by sucrose gradient analysis. With a given virus preparation the method gives highly reproducible results. Typical standard deviations for the 50% breakdown point were about 0.5 degrees C. However, the results are influenced by the method of purification of the virus. Virus purified by CsCl gradient centrifugation was less stable than virus purified on sucrose gradients. Thus the environmental conditions through which the virus passes during processing may affect its stability.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Studies on the stability of foot-and-mouth disease virus using absorbance - temperature profiles. 632 54