Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.9 (
ribonuclease
)
6,589
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Piriformospora indica
, an endophytic root-colonizing fungus, efficiently promotes plant growth and induces resistance to abiotic stress and biotic diseases.
P. indica
fungal cell wall extract induces cytoplasmic calcium elevation in host plant roots. Here, we show that cellotriose (CT) is an elicitor-active cell wall moiety released by
P. indica
into the medium. CT induces a mild defense-like response, including the production of reactive oxygen species, changes in membrane potential, and the expression of genes involved in growth regulation and root development. CT-based cytoplasmic calcium elevation in Arabidopsis (
Arabidopsis thaliana
) roots does not require the
BAK1
coreceptor or the putative Ca
2+
channels TPC1, GLR3.3, GLR2.4, and GLR2.5 and operates synergistically with the elicitor chitin. We identified an ethyl methanesulfonate-induced mutant (
cytoplasmic
calcium elevation mutant
) impaired in the response to CT and various other cellooligomers (
n
= 2-7), but not to chitooligomers (
n
= 4-8), in roots. The mutant contains a single nucleotide exchange in the gene encoding a poly(A)
ribonuclease
(AtPARN; At1g55870) that degrades the poly(A) tails of specific mRNAs. The wild-type
PARN
cDNA, expressed under the control of a 35S promoter, complements the mutant phenotype. Our identification of cellotriose as a novel chemical mediator casts light on the complex
P. indica
-plant mutualistic relationship.
...
PMID:A Poly(A) Ribonuclease Controls the Cellotriose-Based Interaction between
Piriformospora indica
and Its Host Arabidopsis. 2937 Dec 49
Filamentous fungal pathogens secrete effectors that modulate host immunity and facilitate infection. Fusarium graminearum is an important plant pathogen responsible for various devastating diseases. However, little is known about the function of effector proteins secreted by F. graminearum. Herein, we identified several effector candidates in the F. graminearum secretome. Among them, the secreted
ribonuclease
Fg12 was highly upregulated during the early stages of F. graminearum infection in soybean; its deletion compromised the virulence of F. graminearum. Transient expression of Fg12 in Nicotiana benthamiana induced cell death in a light-dependent manner. Fg12 possessed
ribonuclease
(
RNase
) activity, degrading total RNA. The enzymatic activity of Fg12 was required for its cell death-promoting effects. Importantly, the ability of Fg12 to induce cell death was independent of
BAK1
/SOBIR1, and treatment of soybean with recombinant Fg12 protein induced resistance to various pathogens, including F. graminearum and Phytophthora sojae. Overall, our results provide evidence that
RNase
effectors not only contribute to pathogen virulence but also induce plant cell death.
...
PMID:Fg12 ribonuclease secretion contributes to Fusarium graminearum virulence and induces plant cell death. 3272 38
