Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.5 (
RNase P
)
1,348
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
N
6
-methyladenosine (m
6
A) is the most abundant internal modification in RNAs and plays regulatory roles in a variety of biological and physiological processes. Despite its important roles, the molecular mechanism underlying m
6
A-mediated gene regulation is poorly understood. Here, we show that m
6
A-containing RNAs are subject to endoribonucleolytic cleavage via YTHDF2 (m
6
A reader protein), HRSP12 (
adaptor protein
), and
RNase P
/MRP (endoribonucleases). We demonstrate that HRSP12 functions as an adaptor to bridge YTHDF2 and
RNase P
/MRP, eliciting rapid degradation of YTHDF2-bound RNAs. Transcriptome-wide analyses show that m
6
A RNAs that are preferentially targeted for endoribonucleolytic cleavage have an HRSP12-binding site and a
RNase P
/MRP-directed cleavage site upstream and downstream of the YTHDF2-binding site, respectively. We also find that a subset of m
6
A-containing circular RNAs associates with YTHDF2 in an HRSP12-dependent manner and is selectively downregulated by
RNase P
/MRP. Thus, our data expand the known functions of
RNase P
/MRP to endoribonucleolytic cleavage of m
6
A RNAs.
...
PMID:Endoribonucleolytic Cleavage of m
6
A-Containing RNAs by RNase P/MRP Complex. 3093 54
