Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.5 (
RNase P
)
1,348
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Deletion derivatives of C5 protein, the protein cofactor of Escherichia coli
RNase P
, were constructed as soluble
MBP
(maltose-binding protein) fusion proteins to assess the deletion effects on promoting
RNase P
catalysis and on binding to M1 RNA, the catalytic subunit of the enzyme. The C5 protein, with large terminal deletions, retained its promoting activity of
RNase P
catalysis under protein excess conditions in vitro. Some deletion derivatives complemented the temperature sensitive phenotype of E. coli A49 cells carrying the rnpA49 mutation. This ability also suggests that part of the C5 protein is enough to produce the catalytic activity of
RNase P
in vivo. Both the central conserved region, called the RNR motif, and the C-terminal region are essential for the binding of C5 protein to M1 RNA. Meanwhile, the N-terminal region contributes to promoting
RNase P
catalysis in ways other than binding to M1 RNA.
...
PMID:Effects of terminal deletions in C5 protein on promoting RNase P catalysis. 1065 23
