Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.5 (
RNase P
)
1,348
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of macrolide antibiotic spiramycin on
RNase P
holoenzyme and M1 RNA from Escherichia coli was investigated. Ribonuclease P (
RNase P
) is a ribozyme that is responsible for the maturation of 5' termini of tRNA molecules.
Spiramycin
revealed a dose-dependent activation on pre-tRNA cleavage by E. coli
RNase P
holoenzyme and M1 RNA. The K s and V max, as well as the K s(app) and V max(app) values of
RNase P
holoenzyme and M1 RNA in the presence or absence of spiramycin, were calculated from primary and secondary kinetic plots. It was found that the activity status of
RNase P
holoenzyme and M1 RNA is improved by the presence of spiramycin 18- and 12-fold, respectively. Primer extension analysis revealed that spiramycin induces a conformational change of the P10/11 structural element of M1 RNA, which is involved in substrate recognition.
...
PMID:Activation of bacterial ribonuclease P by macrolides. 1833 Sep 98
