Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.5 (
RNase P
)
1,348
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ribonuclease P (
RNase P
) is ubiquitous and essential Mg(2+)-dependent endoribonuclease that catalyzes the 5'-maturation of transfer RNAs.
RNase P
and the ribosome are so far the only ribozymes known to be conserved in all kingdoms of life. Eukaryotic
RNase P
activity has been detected in nuclei, mitochondria and chloroplasts and demonstrates great variability in sequence and subunit composition. In the last few years we have developed methodologies and pursued projects addressing the occurrence, distribution and the potential physiological role of
RNase P
in human epidermal keratinocytes. In view of the vital importance of lymphocytes for an effective immune system and their successful application after transfection with
RNase P
-associated external guide sequences in gene therapy, we concerned ourselves with the isolation and characterization of
RNase P
of peripheral human lymphocytes. We developed a method described herein, that will enable the study of the possible involvement of this ribozyme in the pathogenetic mechanisms of diverse autoimmune, inflammatory and neoplastic cutaneous disorders and may facilitate the further development of
RNase P
-based technology for gene therapy of infectious and neoplastic dermatoses.
Exp
Dermatol
2009 Feb
PMID:Partial purification and characterization of RNase P from human peripheral lymphocytes. 1863 27
