Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.5 (
RNase P
)
1,348
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Measles and
rubella
virus cause fever rash diseases that are uneasy to differentiate clinically from each other. Specific primers and fluorescence-labeled probes were designed, and a multiplex Real-time RT-PCR with an internal control was developed to simultaneously identify the measles and
rubella
virus. The multiplex Real-time RT-PCR assay was specific and no false positive or false negative results were found. The sensitivity of the assay was 0.1TCID50/mL and 1TCID50/mL for measles and
rubella
virus respectively. Analysis with 0.1-10(3)TCID50/mL measles or
rubella
virus samples demonstrated high validity and reproducibility with the coefficient of variability(CV) of below 0.9% for both measles and
rubella
virus. Using
ribonuclease P
(
RNase P
) as internal false negative control, the developed multiplex Real-time RT-PCR assay is suitable for rapid clinical diagnosis of measles and
rubella
virus.
...
PMID:[Simultaneous detection of measles and rubella virus by multiplex real-time RT-PCR with an internal control]. 2048 Jun 39
