Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using
RNase H
is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the
Insulin-like growth factor II receptor
gene which has the area called the GC-block of about 90% GC contents at the 5' terminal. Furthermore, the
RNase H
treatment improved the sensitivity of RT-PCR amplification against a general target.
...
PMID:Improvement of reverse transcription PCR by RNase H. 1464 13
