Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.1.26.4 (
RNase H
)
2,751
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We have analyzed the coding capacity of
B19
parvovirus transcripts by in vitro translation using the negative hybrid selection technique. Five different antisense oligonucleotides (18-mers) corresponding to different portions of the
B19
genome were hybridized to RNA samples extracted from human erythroid bone marrow cells infected with
B19
parvovirus in vitro, and
RNase H
was added to cleave specific
B19
RNA molecules at selected sites.
B19
-specific translation products of these RNA samples were determined by immunoprecipitation. We localized the
B19
nonstructural protein to the left-side transcript and the two capsid proteins to overlapping transcripts from the right side of the genome.
...
PMID:Functional mapping of the genome of the B19 (human) parvovirus by in vitro translation after negative hybrid selection. 337 76
